Is neurotransmitter release involved in the mechanism of general anesthesia?
Is neurotransmitter release involved in the mechanism of general anesthesia?
复制标题
全身麻醉的机制是否涉及神经递质释放？
DOI：
10.1007/s00540-019-02718-1
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发表时间：
2020
影响因子：
2.8
通讯作者：
Kudo Mihoko
中科院分区：
文献类型：
--
作者：
Hirota Kazuyoshi;Kushikata Tetsuya;Takekawa Daiki;Kudo Mihoko
在成年大鼠中，神经递质的释放被认为是通过多种受体系统调节全身麻醉的，这些受体系统是麻醉剂的分子靶点。然而，中年大鼠很少被使用。因此，我们在这个年龄组进行了研究。材料和方法经过批准，我们的协议由机构委员会对动物研究，116中年SD大鼠被分配到氯胺酮（K：n= 74）和丙泊酚（P：n= 42）麻醉组。分别在ip K（100 mg/kg）或P（80 mg/kg）后0、20、60和120 min断头处死大鼠。黑色素浓集激素（MCH），食欲素A（OXA）和去甲肾上腺素的含量在脑桥，下丘脑，海马和大脑皮层的商业酶联免疫吸附测定（ELISA）或高效液相chromatography. ResultsNeurontransmitter含量在所有脑区没有显着改变K或P administration.ConclusionTherefore，我们的问题是否神经递质释放有助于全身麻醉。
IntroductionIt is believed that neurotransmitters release modulates general anesthesia via several receptors system which are molecular targets for anesthetic agents in young-adult rats. However, middle-aged rats have rarely been used. Therefore, we studied in this age group.Materials and MethodsAfter approval of our protocol by the institutional committee on animal research, 116 middle aged Sprague–Dawley rats were assigned to ketamine (K:n= 74) and propofol (P:n= 42) anesthesia groups. Rats were decapitated 0, 20 60 and 120 min after ip K (100 mg/kg) or P (80 mg/kg), respectively. Melanin-concentrating hormone (MCH), orexin A (OXA) and noradrenaline contents in the pons, hypothalamus, hippocampus and cerebrocortex were measured by a commercial enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography.ResultsNeurotransmitter content in all brain regions did not significantly change following K or P administration.ConclusionTherefore, we question whether neurotransmitter release contributes to general anesthesia.
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影响因子：
3.3
作者：
Kushikata, T;Hirota, K;Matsuki, A
通讯作者：
Matsuki, A
影响因子：
2.9
作者：
Hirota, K;Kushikata, T;Matsuki, A
通讯作者：
Matsuki, A
中枢去甲肾上腺素能神经元和全身麻醉的机制。
影响因子：
9.8
作者：
K. Hirota;T. Kushikata
通讯作者：
T. Kushikata
影响因子：
5.7
作者：
Tose, Ryuji;Kushikata, Tetsuya;Hirota, Kazuyoshi
通讯作者：
Hirota, Kazuyoshi