Formation and Expansion of Memory B Cells against Coronavirus in Acutely Infected COVID-19 Individuals.
Formation and Expansion of Memory B Cells against Coronavirus in Acutely Infected COVID-19 Individuals.
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DOI：
10.3390/pathogens11020186
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发表时间：
2022-01-29
期刊：
影响因子：
--
通讯作者：
Topham DJ
中科院分区：
文献类型：
--
作者：
Embong AK;Nguyen-Contant P;Wang J;Kanagaiah P;Chaves FA;Fitzgerald TF;Zhou Q;Kosoy G;Branche AR;Miller BL;Zand MS;Sangster MY;Topham DJ
感染β冠状病毒SARS-CoV-2通常会产生强烈的病毒特异性抗体。针对SARS-CoV-2蛋白新特征的抗体应答需要naïve B细胞激活，但人们越来越认识到，保守区域是由地方性冠状病毒产生的预先存在的记忆B细胞（MBCs）识别的。目前的研究调查了预先存在的交叉反应性冠状病毒记忆在SARS-CoV-2感染后抗体对病毒刺突(S)和核衣壳(N)蛋白的反应中的作用。分析循环抗体、血浆母细胞和红细胞的反应性广度。急性感染受试者对S蛋白产生强烈的IgG反应，包括新的受体结合域、保守的S2区域和N蛋白。这种反应包括对地方性β-冠状病毒的S的反应，有趣的是，对地方性α-冠状病毒的N的反应。轻度和重度感染均增加了对SARS-CoV-2和地方性β-冠状病毒S反应的IgG MBC群体。感染后s反应性IgG抗体和MBCs的活跃度升高。总体而言，研究结果表明，对SARS-CoV-2的S和N的反应涉及预先存在的MBC激活和对蛋白质新特征的适应，以及印记可能影响对SARS-CoV-2感染的反应。
Infection with the β-coronavirus SARS-CoV-2 typically generates strong virus-specific antibody production. Antibody responses against novel features of SARS-CoV-2 proteins require naïve B cell activation, but there is a growing appreciation that conserved regions are recognized by pre-existing memory B cells (MBCs) generated by endemic coronaviruses. The current study investigated the role of pre-existing cross-reactive coronavirus memory in the antibody response to the viral spike (S) and nucleocapsid (N) proteins following SARS-CoV-2 infection. The breadth of reactivity of circulating antibodies, plasmablasts, and MBCs was analyzed. Acutely infected subjects generated strong IgG responses to the S protein, including the novel receptor binding domain, the conserved S2 region, and to the N protein. The response included reactivity to the S of endemic β-coronaviruses and, interestingly, to the N of an endemic α-coronavirus. Both mild and severe infection expanded IgG MBC populations reactive to the S of SARS-CoV-2 and endemic β-coronaviruses. Avidity of S-reactive IgG antibodies and MBCs increased after infection. Overall, findings indicate that the response to the S and N of SARS-CoV-2 involves pre-existing MBC activation and adaptation to novel features of the proteins, along with the potential of imprinting to shape the response to SARS-CoV-2 infection.
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影响因子：
64.8
作者：
Barnes CO;Jette CA;Abernathy ME;Dam KA;Esswein SR;Gristick HB;Malyutin AG;Sharaf NG;Huey-Tubman KE;Lee YE;Robbiani DF;Nussenzweig MC;West AP Jr;Bjorkman PJ
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Bjorkman PJ
影响因子：
3.9
作者：
Cueno ME;Imai K
通讯作者：
Imai K
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16.6
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通讯作者：
Trkola A
影响因子：
6.4
作者：
Guthmiller JJ;Stovicek O;Wang J;Changrob S;Li L;Halfmann P;Zheng NY;Utset H;Stamper CT;Dugan HL;Miller WD;Huang M;Dai YN;Nelson CA;Hall PD;Jansen M;Shanmugarajah K;Donington JS;Krammer F;Fremont DH;Joachimiak A;Kawaoka Y;Tesic V;Madariaga ML;Wilson PC
通讯作者：
Wilson PC
影响因子：
56.9
作者：
Hsieh, Ching-Lin;Goldsmith, Jory A.;McLellan, Jason S.
通讯作者：
McLellan, Jason S.