A Novel 3D Helical Microelectrode Array for In Vitro Extracellular Action Potential Recording.
A Novel 3D Helical Microelectrode Array for In Vitro Extracellular Action Potential Recording.
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一种用于体外细胞外动作电势记录的新型3D螺旋微电极阵列。
DOI：
10.3390/mi13101692
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发表时间：
2022-10-08
期刊：
影响因子：
3.4
通讯作者：
中科院分区：
文献类型：
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作者：
细胞和组织工程学的最新进展已经使人源性神经元组织的长期三维（3D）体外培养成为可能。几十年来，类似的二维（2D）组织培养已与基板集成微电极阵列（MEA）结合用于药理学和毒理学评估。虽然3D培养的表型和细胞结构参数是明确的，但3D MEA技术目前还不够。这主要是由于使用标准化的生物相容性材料和制造技术来创建垂直导电路径（或“迹线”）的技术挑战。在这里，我们通过设计和制造由聚酰亚胺、非晶碳化硅（a-SiC）、金/钛和溅射氧化铱膜（SIROF）组成的新型螺旋3D MEA来规避这一挑战。电化学阻抗谱（EIS）和循环伏安法（CV）测试证实，完全制造的MEA应该能够记录细胞外动作电位（EAP）与高信噪比（SNR）。然后，我们将诱导多能干细胞（iPSC）感觉神经元（SN）接种在与螺旋MEA整合的3D胶原基水凝胶中，并在体外记录来自MEA体积的EAP长达28天。重要的是，这种高度适应性的设计本质上不会限制细胞/组织类型、通道数量、高度或总体积。
Recent advances in cell and tissue engineering have enabled long-term three-dimensional (3D) in vitro cultures of human-derived neuronal tissues. Analogous two-dimensional (2D) tissue cultures have been used for decades in combination with substrate integrated microelectrode arrays (MEA) for pharmacological and toxicological assessments. While the phenotypic and cytoarchitectural arguments for 3D culture are clear, 3D MEA technologies are presently inadequate. This is mostly due to the technical challenge of creating vertical electrical conduction paths (or ‘traces’) using standardized biocompatible materials and fabrication techniques. Here, we have circumvented that challenge by designing and fabricating a novel helical 3D MEA comprised of polyimide, amorphous silicon carbide (a-SiC), gold/titanium, and sputtered iridium oxide films (SIROF). Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) testing confirmed fully-fabricated MEAs should be capable of recording extracellular action potentials (EAPs) with high signal-to-noise ratios (SNR). We then seeded induced pluripotent stems cell (iPSC) sensory neurons (SNs) in a 3D collagen-based hydrogel integrated with the helical MEAs and recorded EAPs for up to 28 days in vitro from across the MEA volume. Importantly, this highly adaptable design does not intrinsically limit cell/tissue type, channel count, height, or total volume.
影响因子：
3.4
作者：
Rihani RT;Kim H;Black BJ;Atmaramani R;Saed MO;Pancrazio JJ;Ware TH
通讯作者：
Ware TH