A conserved homeobox transcription factor Htf1 is required for phialide development and conidiogenesis in Fusarium species.
A conserved homeobox transcription factor Htf1 is required for phialide development and conidiogenesis in Fusarium species.
复制标题
镰刀菌种的花瓶发育和分生孢子发生需要保守的同源盒转录因子 htf1。
DOI：
10.1371/journal.pone.0045432
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Zheng W;Zhao X;Xie Q;Huang Q;Zhang C;Zhai H;Xu L;Lu G;Shim WB;Wang Z
分生孢子是多种病原真菌无性繁殖和传播的主要手段，在动植物疫病传播中起着重要作用。然而，与分生孢子发生相关的遗传机制是复杂的，在许多致病真菌中仍未明确。我们之前的研究表明，水稻稻瘟病菌（Magnaporthe oryzae）的分生条件发生需要同源盒转录因子Htf1。在这项研究中，我们的目的是表征Htf1同源物如何调节三种关键镰刀菌病原体：F. graminearm， F. verticillioides和F. oxysporum的常见和独特的条件发生。与野生型祖先相比，镰刀菌种的基因缺失突变体不能形成常规的philides。相反，它们形成了类似于细长菌丝片段的异常花柱簇，可以想象，这导致了花柱分生的阻碍。我们还观察到，突变体以及野生型镰刀菌可以通过类似发芽的机制直接从菌丝开始产生替代的大分生孢子，尽管频率很低。显微镜观察使我们得出结论，大分生孢子正常的基底细胞分裂和随后的足细胞发育在突变体中受到负面影响。在F. verticillioides和F. oxysporum中，突变体在单孢子顶端有2- 5个微分生孢子复合体，形成花瓣状。此外，在黄萎病菌突变体中没有典型的微分生孢子链。稻谷镰刀菌和黄萎病镰刀菌突变体通过引入其原生HTF1基因或其他镰刀菌的同源物进行补充。这些结果表明，镰刀菌Htf1是一种功能保守的同源盒转录因子，它通过不同的信号通路调节菌体发育和孢子发生，但尚未在真菌中被表征。
Conidia are primary means of asexual reproduction and dispersal in a variety of pathogenic fungi, and it is widely recognized that they play a critical role in animal and plant disease epidemics. However, genetic mechanisms associated with conidiogenesis are complex and remain largely undefined in numerous pathogenic fungi. We previously showed that Htf1, a homeobox transcription factor, is required for conidiogenesis in the rice pathogen Magnaporthe oryzae. In this study, our aim was to characterize how Htf1 homolog regulates common and also distinctive conidiogenesis in three key Fusarium pathogens: F. graminearm, F. verticillioides, and F. oxysporum. When compared to wild-type progenitors, the gene-deletion mutants in Fusarium species failed to form conventional phialides. Rather, they formed clusters of aberrant phialides that resembled elongated hyphae segments, and it is conceivable that this led to the obstruction of conidiation in phialides. We also observed that mutants, as well as wild-type Fusaria, can initiate alternative macroconidia production directly from hyphae through budding-like mechanism albeit at low frequencies. Microscopic observations led us to conclude that proper basal cell division and subsequent foot cell development of macroconidia were negatively impacted in the mutants. In F. verticillioides and F. oxysporum, mutants exhibited a 2- to 5- microconidia complex at the apex of monophialides resulting in a floral petal-like shape. Also, prototypical microconidia chains were absent in F. verticillioides mutants. F. graminearum and F. verticillioides mutants were complemented by introducing its native HTF1 gene or homologs from other Fusarium species. These results suggest that Fusarium Htf1 is functionally conserved homeobox transcription factor that regulates phialide development and conidiogenesis via distinct signaling pathways yet to be characterized in fungi.
登录
查看更多内容
影响因子：
2.8
作者：
Choi, Yoon-E;Shim, Won-Bo
通讯作者：
Shim, Won-Bo
影响因子：
3.7
作者：
Coppin E;Berteaux-Lecellier V;Bidard F;Brun S;Ruprich-Robert G;Espagne E;Aït-Benkhali J;Goarin A;Nesseir A;Planamente S;Debuchy R;Silar P
通讯作者：
Silar P
影响因子：
3.6
作者：
Frandsen, Rasmus J. N.;Nielsen, Nikoline J.;Giese, Henriette
通讯作者：
Giese, Henriette
影响因子：
3.5
作者：
Choi, Yoon-E;Xu, Jin-Rong
通讯作者：
Xu, Jin-Rong
影响因子：
--
作者：
Ding, Shengli;Mehrabi, Rahim;Xu, Jin-Rong
通讯作者：
Xu, Jin-Rong