Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body.
Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body.
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DOI：
10.1371/journal.pone.0037488
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Silar P
中科院分区：
文献类型：
--
作者：
Coppin E;Berteaux-Lecellier V;Bidard F;Brun S;Ruprich-Robert G;Espagne E;Aït-Benkhali J;Goarin A;Nesseir A;Planamente S;Debuchy R;Silar P
高等真菌包括子囊菌和担子菌，在生物圈中起着重要作用。它们的进化成功可能归因于其生命周期中延长的双核阶段，这也是它们学名“双核菌亚界（Dikarya）”的由来。双核状态由相似的结构维持，担子菌中的锁状联合和子囊菌中的产囊丝钩。在所研究的所有担子菌中，同源结构域转录因子是锁状联合形成所必需的。我们鉴定了丝状子囊菌波氏足肿菌（Podospora anserina）中的所有同源框基因，并构建了这些基因以及一些基因组合的缺失突变体。产囊丝钩在这些突变体中正常发育，包括那些多达六个同源基因缺失的突变体。然而，一些突变体在子实体成熟方面存在缺陷，通过提供野生型母本菌丝可以挽救这种影响。对多个同源基因缺陷突变体的分析揭示了基因之间的相互作用，表明它们作为一个复杂的网络发挥作用。与它们在动物和植物中的作用类似，子囊菌中的同源结构域转录因子参与塑造多细胞结构。
Higher fungi, which comprise ascomycetes and basidiomycetes, play major roles in the biosphere. Their evolutionary success may be due to the extended dikaryotic stage of their life cycle, which is the basis for their scientific name: the Dikarya. Dikaryosis is maintained by similar structures, the clamp in basidiomycetes and the crozier in ascomycetes. Homeodomain transcription factors are required for clamp formation in all basidiomycetes studied. We identified all the homeobox genes in the filamentous ascomycete fungus Podospora anserina and constructed deletion mutants for each of these genes and for a number of gene combinations. Croziers developed normally in these mutants, including those with up to six deleted homeogenes. However, some mutants had defects in maturation of the fruiting body, an effect that could be rescued by providing wild-type maternal hyphae. Analysis of mutants deficient in multiple homeogenes revealed interactions between the genes, suggesting that they operate as a complex network. Similar to their role in animals and plants, homeodomain transcription factors in ascomycetes are involved in shaping multicellular structures.
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影响因子：
4.5
作者：
Kim S;Park SY;Kim KS;Rho HS;Chi MH;Choi J;Park J;Kong S;Park J;Goh J;Lee YH
通讯作者：
Lee YH
影响因子：
12.3
作者：
Espagne E;Lespinet O;Malagnac F;Da Silva C;Jaillon O;Porcel BM;Couloux A;Aury JM;Ségurens B;Poulain J;Anthouard V;Grossetete S;Khalili H;Coppin E;Déquard-Chablat M;Picard M;Contamine V;Arnaise S;Bourdais A;Berteaux-Lecellier V;Gautheret D;de Vries RP;Battaglia E;Coutinho PM;Danchin EG;Henrissat B;Khoury RE;Sainsard-Chanet A;Boivin A;Pinan-Lucarré B;Sellem CH;Debuchy R;Wincker P;Weissenbach J;Silar P
通讯作者：
Silar P
影响因子：
3.6
作者：
Brun, Sylvain;Malagnac, Fabienne;Silar, Philippe
通讯作者：
Silar, Philippe
DOI：
10.1007/bf00284694
发表时间：
1993-11-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
COPPIN, E;ARNAISE, S;PICARD, M
通讯作者：
PICARD, M
影响因子：
64.8
作者：
James, Timothy Y.;Kauff, Frank;Vilgalys, Rytas
通讯作者：
Vilgalys, Rytas