Evolution of the oligopeptide transporter family.
Evolution of the oligopeptide transporter family.
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DOI：
10.1007/s00232-011-9347-9
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发表时间：
2011-03
影响因子：
2.4
通讯作者：
Saier, Milton H., Jr.
中科院分区：
文献类型：
--
作者：
Gomolplitinant, Kenny M.;Saier, Milton H., Jr.
肽和铁铁载体转运蛋白的寡肽转运蛋白 (OPT) 家族包括来自原核生物和真核生物的成员，但在后一个域中分布有限。仅在真菌和植物中发现了真核成员，其具有与真菌蛋白聚集的单一粘菌同源物。所有具有功能特征的真核肽转运蛋白都与系统发育树上已知的铁铁载体转运蛋白分离。原核生物成员分布广泛，源自许多不同的门。尽管它们仅属于铁铁载体细分，但基因组背景分析表明它们中的许多是肽转运蛋白。 OPT 家族蛋白具有 16 个或偶尔 17 个跨膜 α 螺旋片段 (TMS)。我们提供的统计证据表明，16-TMS 拓扑是通过三个连续的重复事件以及随后具有第十七个 TMS 的蛋白质的基因融合事件而产生的。建议的路径如下：2 个 TMS → 4 个 TMS → 8 个 TMS → 16 个 TMS → 17 个 TMS。第十七个 C 末端 TMS 可能只出现过一次，仅在这些同源物的一个系统发育组中被发现。直系学分析表明，一些系统发育簇完全由直系同源物组成，但大多数都经历了混合，表明存在水平转移。在这个家族中，水平基因转移在原核生物中很常见，在真核生物中很少见，并且在原核生物和真核生物之间以及植物和真菌之间基本上不存在。这些观察结果为未来 OPT 家族成员的结构和功能分析提供了指导。本文的在线版本 (doi:10.1007/s00232-011-9347-9) 包含补充材料，可供授权用户使用。
The oligopeptide transporter (OPT) family of peptide and iron-siderophore transporters includes members from both prokaryotes and eukaryotes but with restricted distribution in the latter domain. Eukaryotic members were found only in fungi and plants with a single slime mold homologue clustering with the fungal proteins. All functionally characterized eukaryotic peptide transporters segregate from the known iron-siderophore transporters on a phylogenetic tree. Prokaryotic members are widespread, deriving from many different phyla. Although they belong only to the iron-siderophore subdivision, genome context analyses suggest that many of them are peptide transporters. OPT family proteins have 16 or occasionally 17 transmembrane-spanning α-helical segments (TMSs). We provide statistical evidence that the 16-TMS topology arose via three sequential duplication events followed by a gene-fusion event for proteins with a seventeenth TMS. The proposed pathway is as follows: 2 TMSs → 4 TMSs → 8 TMSs → 16 TMSs → 17 TMSs. The seventeenth C-terminal TMS, which probably arose just once, is found in just one phylogenetic group of these homologues. Analyses for orthology revealed that a few phylogenetic clusters consist exclusively of orthologues but most have undergone intermixing, suggestive of horizontal transfer. It appears that in this family horizontal gene transfer was frequent among prokaryotes, rare among eukaryotes and largely absent between prokaryotes and eukaryotes as well as between plants and fungi. These observations provide guides for future structural and functional analyses of OPT family members. The online version of this article (doi:10.1007/s00232-011-9347-9) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
DOI：
10.1073/pnas.95.15.8625
发表时间：
1998-07-21
影响因子：
11.1
作者：
Hirsch, D;Stahl, A;Lodish, HF
通讯作者：
Lodish, HF
DOI：
10.1007/0-387-25856-6_3
发表时间：
2006-01-01
期刊：
Genetic engineering
影响因子：
--
作者：
Lubkowitz, Mark
通讯作者：
Lubkowitz, Mark
影响因子：
14.9
作者：
Käll L;Krogh A;Sonnhammer EL
通讯作者：
Sonnhammer EL
影响因子：
3.6
作者：
Lubkowitz, MA;Barnes, D;Becker, JM
通讯作者：
Becker, JM