IL-22 exacerbates weight loss in a murine model of chronic pulmonary Pseudomonas aeruginosa infection.
IL-22 exacerbates weight loss in a murine model of chronic pulmonary Pseudomonas aeruginosa infection.
复制标题
IL-22加剧了慢性肺假单胞菌感染的鼠模型中的体重减轻。
DOI：
10.1016/j.jcf.2016.06.008
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Evans TJ
中科院分区：
文献类型：
--
作者：
Bayes HK;Ritchie ND;Ward C;Corris PA;Brodlie M;Evans TJ
白细胞介素（IL）-22是粘膜免疫和组织再生的关键介质，保护免受许多呼吸道病原体的侵害。IL-22是否能保护囊性纤维化（CF）患者免受慢性铜绿假单胞菌（PA）感染尚不清楚。检查移植的CF肺的IL-22产生和免疫定位。使用持续性肺PA感染的鼠模型来检查感染攻击后IL-22的产生。使用IL-22敲除（KO）动物检查IL-22的作用。IL-22在成人CF肺内产生并定位于气道上皮。IL-22由肺部感染后的鼠肺淋巴结细胞产生。在慢性PA感染模型中，不存在IL-22导致急性死亡率、细菌负荷、慢性感染率、组织学变化或嗜酸性炎症无显著差异。然而，IL-22 KO动物在感染后体重减轻较少。IL-22在CF肺中产生，并且响应于PA感染，但在鼠模型中对慢性肺铜绿假单胞菌感染具有保护作用。然而，我们发现了一种新的作用，细胞因子在促进感染相关的体重减轻，一个重要的预后因素，在CF人口。
Interleukin (IL)-22 is a critical mediator of mucosal immunity and tissue regeneration, protecting against a number of respiratory pathogens. Whether IL-22 confers protection against chronic Pseudomonas aeruginosa (PA) infection in cystic fibrosis (CF) is unknown. Explanted CF lungs were examined for IL-22 production and immune-localization. A murine model of persistent pulmonary PA infection was used to examine production of IL-22 following infective challenge. The role of IL-22 was examined using IL-22 knockout (KO) animals. IL-22 is produced within the adult CF lung and localizes to the airway epithelium. IL-22 is produced by murine pulmonary lymph node cells following lung infection. The absence of IL-22 resulted in no significant difference in acute mortality, bacterial burden, chronic infection rates, histological changes or neutrophilic inflammation in the chronic PA infection model. However, IL-22 KO animals lost less weight following infection. IL-22 is produced in the CF lung and in response to PA infection yet is dispensable in protection against chronic pulmonary P. aeruginosa infection in a murine model. However, we identified a novel role for the cytokine in promoting infection-related weight-loss, a significant prognostic factor in the CF population.
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