Unique mouse monoclonal antibodies reactive with maturation‐related epitopes on type VII collagen
Unique mouse monoclonal antibodies reactive with maturation‐related epitopes on type VII collagen
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独特的小鼠单克隆抗体与 VII 型胶原蛋白上的成熟相关表位发生反应
DOI：
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发表时间：
2017
影响因子：
3.6
通讯作者：
T. Hashimoto
中科院分区：
文献类型：
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作者：
T. Hayakawa;Y. Hirako;K. Teye;A. Tsuchisaka;H. Koga;N. Ishii;T. Karashima;Minori Kaneda;Yuka Oyu;C. Tateishi;K. Sugawara;A. Yonamine;S. Shinkuma;H. Shimizu;H. Fukano;K. Shimozato;Ngon T. Nguyen;M. Marinkovich;D. Tsuruta;T. Hashimoto
在这项研究中，我们通过用牛角膜衍生基底膜区（BMZ）组分免疫小鼠，产生了一组新的牛和人VII型胶原（COL 7）单克隆抗体（mAb）。这4种mAb（暂命名为COL 7样mAb）除了正常人皮肤和牛角膜的线性BMZ染色外，还显示斑点状表皮下染色，这是COL 7的特征性免疫荧光特征，但通过体外生化分析显示与COL 7无反应性。利用COL 7样mAb不与小鼠BMZ反应的现象，我们比较了野生型和COL 7拯救的人源化小鼠之间的免疫荧光反应性，发现COL 7样mAb与COL 7拯救的人源化小鼠的BMZ反应。在ELISA中，COL 7样mAb与COL 7的完整三螺旋哺乳动物重组蛋白（RP）反应，但不与细菌RP反应。此外，COL 7样mAb在培养的DJM-1细胞或新生儿患者大疱性皮肤的皮肤基底细胞中均不与COL 7反应。这些结果证实了COL 7样mAb与人和牛COL 7发生反应。认为COL 7样mAb的表位仅存在于从角质形成细胞分泌并沉积至BMZ后的成熟COL 7上，并且在免疫印迹过程中容易被破坏。进一步的研究表明，相关的斑点状表皮下染色与IV型胶原蛋白和弹性蛋白。这些独特的抗COL 7单克隆抗体在正常和疾病的研究中应该是有用的，特别是营养不良性大疱性表皮病，它只产生不成熟的COL 7。
In this study, we generated a new set of monoclonal antibodies (mAbs) to bovine and human type VII collagen (COL7) by immunizing mice with bovine cornea‐derived basement membrane zone (BMZ) fraction. The four mAbs, tentatively named as COL7‐like mAbs, showed speckled subepidermal staining in addition to linear BMZ staining of normal human skin and bovine cornea, a characteristic immunofluorescence feature of COL7, but showed no reactivity with COL7 by in vitro biochemical analyses. Taking advantage of the phenomenon that COL7‐like mAbs did not react with mouse BMZ, we compared immunofluorescence reactivity between wild‐type and COL7‐rescued humanized mice and found that COL7‐like mAbs reacted with BMZ of COL7‐rescued humanized mice. In ELISAs, COL7‐like mAbs reacted with intact triple‐helical mammalian recombinant protein (RP) of COL7 but not with bacterial RP. Furthermore, COL7‐like mAbs did not react with COL7 within either cultured DJM‐1 cells or basal cells of skin of a bullous dermolysis of the newborn patient. These results confirmed that COL7‐like mAbs reacted with human and bovine COL7. The epitopes for COL7‐like mAbs were considered to be present only on mature COL7 after secretion from keratinocytes and deposition to BMZ and to be easily destroyed during immunoblotting procedure. Additional studies indicated association of the speckled subepidermal staining with both type IV collagen and elastin. These unique anti‐COL7 mAbs should be useful in studies of both normal and diseased conditions, particularly dystrophic epidermolysis bullosa, which produces only immature COL7.
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影响因子：
6.5
作者：
Chen, M;Marinkovich, MP;Woodley, DT
通讯作者：
Woodley, DT
使用人类 COL7A1 突变对 Col7a1 破坏的小鼠进行角质形成细胞/成纤维细胞靶向拯救，并生成精确的营养不良性大疱性表皮松解症模型。
DOI：
10.2353/ajpath.2009.090347
发表时间：
2009
期刊：
The American journal of pathology
影响因子：
--
作者：
Ito,Kei;Sawamura,Daisuke;Goto,Maki;Nakamura,Hideki;Nishie,Wataru;Sakai,Kaori;Natsuga,Ken;Shinkuma,Satoru;Shibaki,Akihiko;Uitto,Jouni;Denton,ChristopherP;Nakajima,Osamu;Akiyama,Masashi;Shimizu,Hiroshi
通讯作者：
Shimizu,Hiroshi
VII 型胶原蛋白的大非胶原结构域 (NC-1) 是氨基末端且嵌合的。
影响因子：
3.5
作者：
Christiano,AM;Rosenbaum,LM;Chung-Honet,LC;Parente,MG;Woodley,DT;Pan,TC;Zhang,RZ;Chu,ML;Burgeson,RE;Uitto,J
通讯作者：
Uitto,J
大疱性表皮松解症的营养不良和连接形式的分子基础：VII 型胶原蛋白和加里宁（层粘连蛋白 5）基因的突变。
DOI：
10.1111/1523-1747.ep12398967
发表时间：
1994
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Uitto,J;Pulkkinen,L;Christiano,AM
通讯作者：
Christiano,AM