LncRNA Functional Screening in Organismal Development.
LncRNA Functional Screening in Organismal Development.
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DOI：
10.3390/ncrna9040036
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发表时间：
2023-06-28
期刊：
影响因子：
4.3
通讯作者：
Zheng, Ke
中科院分区：
文献类型：
--
作者：
Li, Yang;Zhai, Huicong;Tong, Lingxiu;Wang, Cuicui;Xie, Zhiming;Zheng, Ke
尽管长链非编码 RNA (lncRNA) 在各种细胞和生物体中得到了广泛研究，但其功能普遍性仍存在争议。在动物中，lncRNA 引起了普遍的兴趣，因为转录组库呈指数增长，报告了它们的高度组织特异性和发育动态表达，更重要的是，越来越多的实验证据支持它们在促进器官发生和个体适应性方面的功能。在哺乳动物睾丸中，虽然鉴定出了大量的 lncRNA 种类，但只有少数种类被证明是有用的，而且迄今为止使用敲除模型被证明是男性生育能力的真正要求的种类就更少了。这种显着的差距归因于大量垃圾lncRNA的虚拟存在、缺乏理想的种系培养系统、功能丧失询问的困难以及有限的筛选策略。面对这些挑战，在这篇综述中，我们讨论了lncRNA在生物体发育中的功能，特别是在小鼠睾丸中，重点是lncRNA的功能筛选。
Controversy continues over the functional prevalence of long non-coding RNAs (lncRNAs) despite their being widely investigated in all kinds of cells and organisms. In animals, lncRNAs have aroused general interest from exponentially increasing transcriptomic repertoires reporting their highly tissue-specific and developmentally dynamic expression, and more importantly, from growing experimental evidence supporting their functionality in facilitating organogenesis and individual fitness. In mammalian testes, while a great multitude of lncRNA species are identified, only a minority of them have been shown to be useful, and even fewer have been demonstrated as true requirements for male fertility using knockout models to date. This noticeable gap is attributed to the virtual existence of a large number of junk lncRNAs, the lack of an ideal germline culture system, difficulty in loss-of-function interrogation, and limited screening strategies. Facing these challenges, in this review, we discuss lncRNA functionality in organismal development and especially in mouse testis, with a focus on lncRNAs with functional screening.
影响因子：
5.2
作者：
Werner A;Piatek MJ;Mattick JS
通讯作者：
Mattick JS