Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1
Human monocytes bind to two cytokine-induced adhesive ligands on cultured human endothelial cells: endothelial-leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1
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人单核细胞与培养的人内皮细胞上的两个细胞因子诱导的粘附配体结合：内皮白细胞粘附分子-1和血管细胞粘附分子-1
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
J. Harlan
中科院分区：
文献类型：
--
作者：
T. Carlos;N. Kovach;B. Schwartz;M. Rosa;B. Newman;E. Wayner;C. Benjamin;L. Osborn;R. Lobb;J. Harlan
血管细胞黏附分子-1 （VCAM-1）和内皮-白细胞黏附分子-1 （ELAM-1）是细胞因子诱导的内皮细胞黏附蛋白。我们使用转染的中国仓鼠卵巢（CHO）细胞稳定表达这些粘附蛋白和针对ELAM-1、VCAM-1或CD49d/CD29 （VCAM-1的白细胞受体）的单克隆抗体（MoAbs），研究了这些粘附蛋白对人外周血单核细胞粘附内皮的贡献。单核细胞与转染了ELAM-1或VCAM-1 cDNA的CHO细胞结合。MoAb抑制了与ELAM-1的结合，MoAb抑制了与VCAM-1或极晚活化抗原-4 (VLA-4) α链（CD49d）的结合。用MoAb对CD49d和MoAb对CD18（白细胞β 2整合素受体的共同β链）预处理单核细胞，观察到对未刺激的人脐静脉内皮（HUVE）粘附的累加性抑制。单独使用CD18、CD49d或ELAM- 1的MoAbs并不会降低重组人肿瘤坏死因子- α刺激下单核细胞对HUVE的粘附，但这些MoAbs联合使用会产生显著的抑制作用。我们得出结论，多个受体配体系统参与单核细胞粘附内皮。
Vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte adhesion molecule-1 (ELAM-1) are adhesive proteins induced on endothelium by cytokines. We examined the contribution of these adhesive proteins to human peripheral blood monocyte adherence to endothelium using transfected Chinese hamster ovary (CHO) cells stably expressing these proteins and monoclonal antibodies (MoAbs) to ELAM-1, VCAM-1, or CD49d/CD29 (VLA-4), the leukocyte receptor for VCAM-1. Monocytes bound to CHO cells transfected with cDNA of ELAM-1 or VCAM-1. Binding to ELAM-1 was inhibited by MoAb to ELAM-1 and binding to VCAM-1 was inhibited by MoAb to VCAM-1 or the alpha-chain of very late activation antigen-4 (VLA-4) (CD49d). Additive inhibition of adherence to unstimulated human umbilical vein endothelium (HUVE) was observed when monocytes were pretreated with both MoAb to CD49d and MoAb to CD18, the common beta-chain of the leukocyte beta 2 integrin receptors. Adherence of monocytes to HUVE stimulated by recombinant human tumor necrosis factor-alpha was not reduced by MoAbs to CD18, CD49d, or ELAM- 1 when used singly, but combinations of these MoAbs produced significant inhibition. We conclude that multiple receptor-ligand systems are involved in monocyte adherence to endothelium.
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肿瘤坏死因子和干扰素-γ 诱导狒狒皮肤中不同的内皮激活模式和相关的白细胞积聚。
DOI：
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发表时间：
1989
期刊：
The American journal of pathology
影响因子：
--
作者：
Munro,JM;Pober,JS;Cotran,RS
通讯作者：
Cotran,RS
DOI：
10.1016/0167-5699(89)90238-7
发表时间：
1989-04-01
期刊：
IMMUNOLOGY TODAY
影响因子：
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作者：
HOGG, N
通讯作者：
HOGG, N
影响因子：
158.5
作者：
JOHNSTON, RB
通讯作者：
JOHNSTON, RB
影响因子：
56.9
作者：
BEVILACQUA, MP;STENGELIN, S;SEED, B
通讯作者：
SEED, B
影响因子：
4.4
作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer
通讯作者：
J. Pober;M. Gimbrone;L. Lapierre;D. Mendrick;W. Fiers;R. Rothlein;T. Springer