Protective effects of Danshen injection against erectile dysfunction via suppression of endoplasmic reticulum stress activation in a streptozotocin-induced diabetic rat model
Protective effects of Danshen injection against erectile dysfunction via suppression of endoplasmic reticulum stress activation in a streptozotocin-induced diabetic rat model
复制标题
丹参注射液通过抑制内质网应激激活对链脲佐菌素诱导的糖尿病大鼠模型勃起功能障碍的保护作用
DOI：
10.1186/s12906-018-2414-3
复制
发表时间：
2018-12
影响因子：
--
通讯作者：
徐麟皓
中科院分区：
文献类型：
--
作者：
张勇;陈健;季华;肖占刚;沈培强;徐麟皓
勃起功能障碍（艾德）是糖尿病的常见并发症。本研究旨在探讨丹参注射液对链脲佐菌素（STZ）诱导的糖尿病大鼠艾德的保护作用及其机制。糖尿病大鼠腹腔注射丹参液（0.5或1 mL/kg/d）或等量生理盐水，连续6周。将配对的大鼠作为对照。6周后，评估勃起功能和阴茎海绵体的组织学形态。测定阴茎组织中的氧化应激指标，包括超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量和活性氧（ROS）水平。采用免疫组化、免疫印迹和RT-PCR检测葡萄糖调节蛋白78（Grp 78）、生长停滞和DNA损伤诱导基因153（GADD 153/CHOP）的表达水平。TUNEL法检测细胞凋亡。结果糖尿病大鼠勃起次数明显少于对照组。丹参注射液可通过增加勃起次数改善勃起功能。丹参注射液还被发现改善阴茎海绵体的形态异常，减少凋亡细胞的数量，并抑制阴茎组织中caspase-3的活化，伴随着内质网应激生物标志物Grp 78和CHOP的下调。结论丹参注射液对糖尿病相关艾德性ED有一定的治疗作用，其机制可能与抑制氧化应激和内质网应激诱导的细胞凋亡有关。
BackgroundErectile dysfunction (ED) is a common complication of diabetes. This study aimed to explore the beneficial effect of Danshen injection on ED in a streptozotocin (STZ)-induced diabetic rat model and the underlying mechanism.MethodsThe diabetic rat model was established by an intraperitoneal injection of 60 mg/kg STZ in male Sprague-Dawley rats. The diabetic rats were intraperitoneally injected with Danshen solution (0.5 or 1 mL/kg/day) or the same volume of saline for 6 weeks. Age-matched rats served as controls. After 6 weeks, erectile function and histological morphology of the corpora cavernosum were assessed. Oxidative stress indicators, including superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and reactive oxygen species (ROS) levels, were measured in penile tissues. The expression levels of glucose-regulated protein 78 (Grp78), growth arrest and DNA damage-inducible gene 153 (GADD153/CHOP) were determined by immunohistochemistry, immunoblotting, and RT-PCR. Apoptosis was detected by a TUNEL assay.ResultsThe erection times of diabetic rats were significantly less than those of control rats. Danshen injection could improve erectile function via increased erection times. Danshen injection was also found to ameliorate the morphological abnormalities of the corpora cavernosum, to reduce the number of apoptotic cells, and to suppress caspase-3 activation in penile tissue, accompanied by downregulation of the endoplasmic reticulum stress biomarkers Grp78 and CHOP. Danshen injection could increase SOD activity as well as reduce ROS and MDA levels in diabetic rats, indicating suppression of oxidative stress.ConclusionDanshen injection could rescue diabetes-associated ED, possibly via suppressing the oxidative stress and endoplasmic reticulum (ER) stress-induced apoptosis pathways.
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影响因子：
5.6
作者：
Xu, Linhao;Shen, Peiqiang;Wang, Zheng
通讯作者：
Wang, Zheng
影响因子：
6.7
作者：
Han-yang Ye;Zhan-yuan Li;Yu Zheng;Yan Chen;Zhi-hong Zhou;Jian Jin
通讯作者：
Han-yang Ye;Zhan-yuan Li;Yu Zheng;Yan Chen;Zhi-hong Zhou;Jian Jin
影响因子：
1.6
作者：
Li, Huan;He, Wei-Yang;Gou, Xin
通讯作者：
Gou, Xin
DOI：
10.1186/s13058-015-0538-7
发表时间：
2015-02-21
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Azim HA Jr;Peccatori FA;Brohée S;Branstetter D;Loi S;Viale G;Piccart M;Dougall WC;Pruneri G;Sotiriou C
通讯作者：
Sotiriou C
影响因子：
1.4
作者：
S. LaVignera
通讯作者：
S. LaVignera