Peripheral Lipopolyssacharide Rapidly Silences REM-Active LH(GABA) Neurons.
Peripheral Lipopolyssacharide Rapidly Silences REM-Active LH(GABA) Neurons.
复制标题
外周脂多糖快速沉默REM活性LH（GABA）神经元。
DOI：
10.3389/fnbeh.2021.649428
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发表时间：
2021
影响因子：
3
通讯作者：
de Lecea L
中科院分区：
文献类型：
--
作者：
Borniger JC;de Lecea L
免疫因素（例如，细胞因子、趋化因子）可以改变神经元回路的活性以促进“病态行为”，这是生物体响应感染/损伤以使其恢复机会最大化而表现出的一系列适应性行为（即，回到稳态）。这包括睡眠/觉醒状态、运动活动和食物摄入等行为的急剧改变。尽管有充分的证据强调大脑和全身免疫之间的相互作用，但关于免疫挑战如何改变控制唤醒状态的遗传定义的细胞群的活动的研究很少。由于外侧下丘脑（LH）在行为唤醒、食物摄入以及监测和响应全身生理学变化方面具有主要的综合功能，因此我们研究了该脑区内的GABA能神经元如何在正常睡眠/觉醒状态下以及响应细菌内毒素[脂多糖（LPS）]的外周免疫挑战时改变其活性。利用纤维光度法（GCaMP 6sCa 2+信号）结合脑电图（EEG）/肌电图记录来确定唤醒状态，我们观察到外侧下丘脑（LHGABA）中GABA能神经元的群体活动在快速眼动睡眠（REM）期间最高，并且这种活动在自发唤醒状态转换期间急剧变化，在非REM睡眠期间观察到最低活动。在腹腔内LPS刺激后，LHGABA神经元迅速降低其活性，同时消除REM睡眠行为（麻黄碱诱导的疾病的特征）。总之，这些数据表明，外周免疫激发可以迅速（< 40分钟）改变控制唤醒状态的皮层下神经元回路。此外，我们证明，纤维光度法提供了一个敏感的和细胞类型的具体工具，可以应用于研究疾病行为的神经元基板。
Immune factors (e.g., cytokines, chemokines) can alter the activity of neuronal circuits to promote “sickness behavior,” a suite of adaptive actions that organisms exhibit in response to infection/injury in order to maximize their chances of recovery (i.e., return to homeostasis). This includes drastic alterations in sleep/wake states, locomotor activity, and food intake, among other behaviors. Despite the ample evidence highlighting interactions between the brain and systemic immunity, studies on how immune challenges alter the activity of genetically defined cell populations controlling arousal states are scarce. As the lateral hypothalamus (LH) serves a major integrative function in behavioral arousal, food intake, and monitoring and responding to changes in systemic physiology, we investigated how GABAergic neurons within this brain region alter their activity across normal sleep/wake states and in response to a peripheral immune challenge with bacterial endotoxin [lipopolysaccharides (LPS)]. Using fiber photometry (GCaMP6s Ca2+ signal) in tandem with electroencephalogram (EEG)/EMG recordings to determine arousal states, we observed that population activity of GABAergic neurons in the lateral hypothalamus (LHGABA) is highest during rapid-eye-movement sleep (REM), and this activity changes drastically across spontaneous arousal state transitions, with the lowest activity observed during non-REM sleep. Upon intraperitoneal LPS challenge, LHGABA neurons rapidly decrease their activity in tandem with elimination of REM sleep behavior (characteristic of cytokine-induced sickness). Together, these data suggest that peripheral immune challenges can rapidly (in < 40 min) alter subcortical neuronal circuits controlling arousal states. Additionally, we demonstrate that fiber photometry offers a sensitive and cell-type specific tool that can be applied to study the neuronal substrates of sickness behavior.
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DOI：
10.1111/j.1460-9568.2010.07295.x
发表时间：
2010-08
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Hassani OK;Henny P;Lee MG;Jones BE
通讯作者：
Jones BE
影响因子：
56.9
作者：
HOEBEL, BG;TEITELBAUM, P
通讯作者：
TEITELBAUM, P
影响因子：
15.1
作者：
Gaykema, Ronald P. A.;Goehler, Lisa E.
通讯作者：
Goehler, Lisa E.
DOI：
10.1073/pnas.1202526109
发表时间：
2012-09-25
影响因子：
11.1
作者：
Carter, Matthew E.;Brill, Julia;de Lecea, Luis
通讯作者：
de Lecea, Luis
影响因子：
25
作者：
Eban-Rothschild A;Rothschild G;Giardino WJ;Jones JR;de Lecea L
通讯作者：
de Lecea L