Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides.
Mnt2p and Mnt3p of Saccharomyces cerevisiae are members of the Mnn1p family of alpha-1,3-mannosyltransferases responsible for adding the terminal mannose residues of O-linked oligosaccharides.
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酿酒酵母的 Mnt2p 和 Mnt3p 是 α-1,3-甘露糖基转移酶 Mnn1p 家族的成员，负责添加 O-连接寡糖的末端甘露糖残基。
作者：
Pedro A. Romero;Marc Lussier;Steeve Veronneau;A. Sdicu;A. Herscovics;H. Bussey
酿酒酵母的基因组含有5个编码II型跨膜蛋白的基因，这些蛋白与α-1，3-甘露糖基转移酶Mnn 1 p具有显著的氨基酸相似性。与MNN 1最密切相关的三个基因的作用在携带单个和多个破坏基因组合的突变体中进行了研究。通过β-消除释放的[2- 3 H]甘露糖标记的O-连接寡糖的纸色谱分析显示，MNT 2（YGL 257 c）和MNT 3（YIL 014 w）基因与MNN 1组合在添加第四和第五α-1，3-连接的甘露糖残基形成Man 4和Man 5寡糖，而MNT 4（YNR 059 w）似乎不是O-聚糖合成所需的。
The genome of Saccharomyces cerevisiae contains five genes that encode type II transmembrane proteins with significant amino acid similarity to the alpha-1,3-mannosyltransferase Mnn1p. The roles of the three genes most closely related to MNN1 were examined in mutants carrying single and multiple combinations of the disrupted genes. Paper chromatographic analysis of [2-3H]mannose-labeled O-linked oligosaccharides released by beta-elimination showed that the MNT2 (YGL257c) and MNT3 (YIL014w) genes in combination with MNN1 have overlapping roles in the addition of the fourth and fifth alpha-1,3-linked mannose residues to form Man4 and Man5 oligosaccharides whereas MNT4 (YNR059w) does not appear to be required for O-glycan synthesis.
DOI：
10.1016/s0021-9258(18)33332-5
发表时间：
1982-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Byrd;A. Tarentino;F. Maley;P. H. Atkinson;R. B. Trimble
通讯作者：
J. Byrd;A. Tarentino;F. Maley;P. H. Atkinson;R. B. Trimble
DOI：
10.1073/pnas.89.15.6846
发表时间：
1992-08-01
影响因子：
11.1
作者：
HAUSLER, A;BALLOU, L;ROBBINS, PW
通讯作者：
ROBBINS, PW