Culture media composition influences patient-derived organoid ability to predict therapeutic responses in gastrointestinal cancers.
Culture media composition influences patient-derived organoid ability to predict therapeutic responses in gastrointestinal cancers.
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DOI：
10.1172/jci.insight.158060
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发表时间：
2022-11-22
期刊：
影响因子：
8
通讯作者：
Fernandez-Zapico, Martin E.
中科院分区：
文献类型：
--
作者：
Hogenson, Tara L.;Xie, Hao;Phillips, William J.;Toruner, Merih D.;Li, Jenny J.;Horn, Isaac P.;Kennedy, Devin J.;Almada, Luciana L.;Marks, David L.;Carr, Ryan M.;Toruner, Murat;Sigafoos, Ashley N.;Koenig-Kappes, Amanda N.;Olson, Rachel L. O.;Tolosa, Ezequiel J.;Zhang, Cheng;Li, Hu;Doles, Jason D.;Bleeker, Jonathan;Barrett, Michael T.;Boyum, James H.;Kipp, Benjamin R.;Mahipal, Amit;Hubbard, Joleen M.;Hanson, Temperance J. Scheffler;Petersen, Gloria M.;Dasari, Surendra;Oberg, Ann L.;Truty, Mark J.;Graham, Rondell P.;Levy, Michael J.;Zhu, Mojun;Billadeau, Daniel D.;Adjei, Alex A.;Dusetti, Nelson;Iovanna, Juan L.;Bekaii-Saab, Tanios S.;Ma, Wen Wee;Fernandez-Zapico, Martin E.
患者源性类器官（PDO）平台可能成为转化性癌症研究的一个有前途的工具。在这项研究中，我们评估了PDO预测胃肠道（GI）癌症临床反应的能力。我们从胃肠道癌症患者的原发性和转移性病变中生成PDOs，包括胰腺导管腺癌、结直肠腺癌和胆管癌。我们比较了PDO反应与供体患者在相同治疗下观察到的临床反应。我们报告了PDO和供体肿瘤反应之间约80%的一致性率。重要的是，我们发现培养基对PDO表型有深远的影响，我们发现在相同的PDO培养中，不同的培养基对标准化疗、不同的形态和转录组的反应有显著差异。虽然我们证明供体肿瘤和PDO之间的一致性很高，但这些研究也表明，在使用PDO来指导治疗选择和预测胃肠道癌症反应时，培养基的重要作用。不适用。结直肠癌研究的Joan F. & Richard A. Abdoo家庭基金，梅奥诊所癌症中心的GI癌症项目，梅奥诊所胰腺癌孢子，个体化医学中心（梅奥诊所），实验室医学和病理学部（梅奥诊所），Incyte制药和梅奥诊所肝胆孢子，明尼苏达大学-梅奥诊所合作伙伴关系，以及早期治疗项目（梅奥诊所肿瘤部）。
A patient-derived organoid (PDO) platform may serve as a promising tool for translational cancer research. In this study, we evaluated PDO’s ability to predict clinical response to gastrointestinal (GI) cancers. We generated PDOs from primary and metastatic lesions of patients with GI cancers, including pancreatic ductal adenocarcinoma, colorectal adenocarcinoma, and cholangiocarcinoma. We compared PDO response with the observed clinical response for donor patients to the same treatments. We report an approximately 80% concordance rate between PDO and donor tumor response. Importantly, we found a profound influence of culture media on PDO phenotype, where we showed a significant difference in response to standard-of-care chemotherapies, distinct morphologies, and transcriptomes between media within the same PDO cultures. While we demonstrate a high concordance rate between donor tumor and PDO, these studies also showed the important role of culture media when using PDOs to inform treatment selection and predict response across a spectrum of GI cancers. Not applicable. The Joan F. & Richard A. Abdoo Family Fund in Colorectal Cancer Research, GI Cancer program of the Mayo Clinic Cancer Center, Mayo Clinic SPORE in Pancreatic Cancer, Center of Individualized Medicine (Mayo Clinic), Department of Laboratory Medicine and Pathology (Mayo Clinic), Incyte Pharmaceuticals and Mayo Clinic Hepatobiliary SPORE, University of Minnesota-Mayo Clinic Partnership, and the Early Therapeutic program (Department of Oncology, Mayo Clinic).
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
64.5
作者：
Lee SH;Hu W;Matulay JT;Silva MV;Owczarek TB;Kim K;Chua CW;Barlow LJ;Kandoth C;Williams AB;Bergren SK;Pietzak EJ;Anderson CB;Benson MC;Coleman JA;Taylor BS;Abate-Shen C;McKiernan JM;Al-Ahmadie H;Solit DB;Shen MM
通讯作者：
Shen MM
影响因子：
4.6
作者：
Kumar K;Raza SS;Knab LM;Chow CR;Kwok B;Bentrem DJ;Popovic R;Ebine K;Licht JD;Munshi HG
通讯作者：
Munshi HG
影响因子：
11.2
作者：
Lenkiewicz E;Malasi S;Hogenson TL;Flores LF;Barham W;Phillips WJ;Roesler AS;Chambers KR;Rajbhandari N;Hayashi A;Antal CE;Downes M;Grandgenett PM;Hollingsworth MA;Cridebring D;Xiong Y;Lee JH;Ye Z;Yan H;Hernandez MC;Leiting JL;Evans RM;Ordog T;Truty MJ;Borad MJ;Reya T;Von Hoff DD;Fernandez-Zapico ME;Barrett MT
通讯作者：
Barrett MT
DOI：
10.1158/1078-0432.ccr-20-4116
发表时间：
2022-02-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Grossman JE;Muthuswamy L;Huang L;Akshinthala D;Perea S;Gonzalez RS;Tsai LL;Cohen J;Bockorny B;Bullock AJ;Schlechter B;Peters MLB;Conahan C;Narasimhan S;Lim C;Davis RB;Besaw R;Sawhney MS;Pleskow D;Berzin TM;Smith M;Kent TS;Callery M;Muthuswamy SK;Hidalgo M
通讯作者：
Hidalgo M