Calcium Signal Profiles in Vascular Endothelium from Cdh5-GCaMP8 and Cx40-GCaMP2 Mice.
Calcium Signal Profiles in Vascular Endothelium from Cdh5-GCaMP8 and Cx40-GCaMP2 Mice.
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DOI：
10.1159/000514210
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发表时间：
2021
影响因子：
1.7
通讯作者：
Sonkusare SK
中科院分区：
文献类型：
--
作者：
Chen YL;Baker TM;Lee F;Shui B;Lee JC;Tvrdik P;Kotlikoff MI;Sonkusare SK
在Cx40-GCaMP2小鼠中，通过连接蛋白40启动子在内皮中表达钙生物传感器GCaMP2，研究发现了内皮钙信号的独特特性。然而，Cx40-GCaMP2小鼠与静脉内皮中狭窄的动态范围和缺乏信号相关。最近的研究提出了许多具有改进性质的gcamp (GCaMP5/6/7/8)，尽管它们在内皮特异性钙研究中的表现尚不清楚。我们鉴定了一种新开发的小鼠系，该系在VE-Cadherin （Cdh5-GCaMP8）启动子下在内皮中组成性地表达GCaMP8。Cdh5-GCaMP8和Cx40-GCaMP2小鼠肠系膜动脉和静脉中通过内皮IP3受体和TRPV4离子通道记录钙信号。Cdh5-GCaMP8小鼠比Cx40-GCaMP2小鼠表现出更低的基线荧光强度、更高的动态范围和更高的单个钙信号振幅。重要的是，Cdh5-GCaMP8小鼠在完整的静脉内皮中首次记录了离散的钙信号，并揭示了肠系膜动脉和静脉之间IP3受体和TRPV4通道钙信号的显著差异。我们的研究结果表明，Cdh5-GCaMP8小鼠在动态范围、对低强度信号的敏感性以及记录静脉内皮钙信号的能力方面表现出显著的改善。
Studies in Cx40-GCaMP2 mice, which express calcium biosensor GCaMP2 in the endothelium under connexin 40 promoter, have identified the unique properties of endothelial calcium signals. However, Cx40-GCaMP2 mouse is associated with a narrow dynamic range and lack of signal in the venous endothelium. Recent studies have proposed many GCaMPs (GCaMP5/6/7/8) with improved properties, although their performance in endothelium-specific calcium studies is not known. We characterized a newly developed mouse line that constitutively expresses GCaMP8 in the endothelium under the VE-Cadherin (Cdh5-GCaMP8) promoter. Calcium signals through endothelial IP3 receptors and TRPV4 ion channels were recorded in mesenteric arteries and veins from Cdh5-GCaMP8 and Cx40-GCaMP2 mice. Cdh5-GCaMP8 mice showed lower baseline fluorescence intensity, higher dynamic range, and higher amplitudes of individual calcium signals than Cx40-GCaMP2 mice. Importantly, Cdh5-GCaMP8 mice enabled the first recordings of discrete calcium signals in the intact venous endothelium and revealed striking differences in IP3 receptor and TRPV4 channel calcium signals between mesenteric arteries and veins. Our findings suggest that Cdh5-GCaMP8 mice represent significant improvements in dynamic range, sensitivity for low-intensity signals, and the ability to record calcium signals in venous endothelium.
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影响因子：
3.7
作者：
Ohkura M;Sasaki T;Sadakari J;Gengyo-Ando K;Kagawa-Nagamura Y;Kobayashi C;Ikegaya Y;Nakai J
通讯作者：
Nakai J
影响因子：
2.5
作者：
Ivanova, Elena;Kovacs-Oller, Tamas;Sagdullaev, Botir T.
通讯作者：
Sagdullaev, Botir T.
影响因子：
20.1
作者：
Francis M;Waldrup JR;Qian X;Solodushko V;Meriwether J;Taylor MS
通讯作者：
Taylor MS
DOI：
10.1002/wsbm.1448
发表时间：
2019-09-01
影响因子：
7.9
作者：
Ottolini, Matteo;Hong, Kwangseok;Sonkusare, Swapnil K.
通讯作者：
Sonkusare, Swapnil K.
DOI：
10.1152/ajpheart.00425.2011
发表时间：
2011-09-01
影响因子：
4.8
作者：
Bagher, Pooneh;Davis, Michael J.;Segal, Steven S.
通讯作者：
Segal, Steven S.