Cholinergic dopamine release from the in vitro rabbit carotid body.
Cholinergic dopamine release from the in vitro rabbit carotid body.
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体外兔颈动脉体释放胆碱能多巴胺。
DOI：
10.1152/jappl.2000.88.5.1737
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发表时间：
2000
影响因子：
3.3
通讯作者：
F. Marchal
中科院分区：
文献类型：
--
作者：
A. Bairam;H. Néji;F. Marchal
本研究的目的是测试胆碱能机制是否调节多巴胺（DA）从颈动脉体（CB）的释放，并与DA D（2）自身受体相互作用。将142只成年家兔的CB在体外注入充满O（2）的存活培养基中（Bairam A，Marchal F，Cottet-Emard JM，Basson H，Pequignot JM，Hascoet JM和Lahiri S. J Appl Physiol 80：20-24，1996）。在暴露于各种处理1小时后测量CB DA含量和释放：对照，胆碱能激动剂（0.1-50 μ M卡巴胆碱），完全毒蕈碱拮抗剂（1和10 μ M阿托品），M（1）和M（2）毒蕈碱受体拮抗剂（分别为1和10 μ M哌仑西平和10 μ M AFDX-116），以及DA D（2）受体拮抗剂多潘立酮（1 μ M），单独使用和与卡巴胆碱（1 μ M）一起使用。与对照组相比，卡巴胆碱（1-50 μ M），AFDX-116和多潘立酮显著增加DA的释放，阿托品（10 μ M）和哌仑西平（10 μ M）减少DA的释放。多潘立酮和卡巴胆碱的效果没有显着差异，但明显相加。结果表明，在兔CB中，除DA D（2）自身受体外，M（1）和M（2）受体亚型可能参与DA释放的调控。
The aim of this study was to test whether cholinergic mechanisms regulate dopamine (DA) release from the carotid body (CB) and interact with DA D(2) autoreceptors. One hundred forty-two CBs from adult rabbits were infused in vitro in a surviving medium bubbled with O(2) (Bairam A, Marchal F, Cottet-Emard JM, Basson H, Pequignot JM, Hascoet JM, and Lahiri S. J Appl Physiol 80: 20-24, 1996). CB DA content and release were measured after 1 h of exposure to various treatments: control, cholinergic agonist (0.1-50 microM carbachol), full muscarinic antagonist (1 and 10 microM atropine), antagonists of M(1) and M(2) muscarinic receptors (1 and 10 microM pirenzepine and 10 microM AFDX-116, respectively), and the DA D(2) receptor antagonist domperidone (1 microM), alone and with carbachol (1 microM). Compared with control, the release of DA was significantly increased by carbachol (1-50 microM), AFDX-116, and domperidone and decreased by atropine (10 microM) and pirenzepine (10 microM). The effects of domperidone and carbachol were not significantly different but were clearly additive. It is concluded that, in the rabbit CB, M(1) and M(2) muscarinic receptor subtypes may be involved in the control of DA release, in addition to the DA D(2) autoreceptors.
猫和兔颈动脉体中的烟碱与毒蕈碱结合位点。
DOI：
10.1159/000109319
发表时间：
1992
期刊：
Biological signals
影响因子：
--
作者：
Hirano,T;Dinger,B;Yoshizaki,K;Gonzalez,C;Fidone,S
通讯作者：
Fidone,S
猫缺氧颈动脉体化学转导的进一步胆碱能方面。
DOI：
10.1152/jappl.1997.82.3.819
发表时间：
1997
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Fitzgerald,RS;Shirahata,M;Ide,T
通讯作者：
Ide,T