Downregulation of barley ferulate 5-hydroxylase dramatically alters straw lignin structure without impact on mechanical properties.
Downregulation of barley ferulate 5-hydroxylase dramatically alters straw lignin structure without impact on mechanical properties.
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DOI：
10.3389/fpls.2022.1125003
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
大麦是温带气候的主要谷类作物，也是多倍体小麦的二倍体遗传模型。谷物秸秆生物质是绿色技术有吸引力的原料来源，但木质素是原料不顺从的关键决定因素，使生物转化过程复杂化。然而，控制木质素含量以改善转化过程可能会对农艺性状产生负面影响。另一种方法是操纵影响秸秆物理和化学性质的木质素成分。这项研究验证了大麦阿魏酸 5-羟化酶基因的功能，并证明使用 RNA 干扰方法对其进行下调会显着影响木质素组成。我们鉴定了五个具有推定阿魏酸 5-羟化酶活性的大麦基因。 HvF5H1的下调显着降低了秸秆中木质素紫丁香基/愈创木基（S/G）的比例，而木质素含量、秸秆机械性能、植物生长习性和谷物特性均不受影响。代谢分析揭示了 HvF5H1-RNAi 系中 173 个特征丰度的显着变化。转基因品系木质素聚合物的巨大变化凸显了大麦木质化过程的可塑性以及操纵和改进木质纤维素生物质作为绿色技术原料的相关潜力。另一方面，我们的结果强调了大麦（温带气候草）和温暖气候草（水稻）的木质素生物合成途径之间的一些差异，并强调了草中木质素生物合成途径的潜在多样性。
Barley is a major cereal crop for temperate climates, and a diploid genetic model for polyploid wheat. Cereal straw biomass is an attractive source of feedstock for green technologies but lignin, a key determinant of feedstock recalcitrance, complicates bio-conversion processes. However, manipulating lignin content to improve the conversion process could negatively affect agronomic traits. An alternative approach is to manipulate lignin composition which influences the physical and chemical properties of straw. This study validates the function of a barley ferulate 5-hydroxylase gene and demonstrates that its downregulation using the RNA-interference approach substantially impacts lignin composition. We identified five barley genes having putative ferulate 5-hydroxylase activity. Downregulation of HvF5H1 substantially reduced the lignin syringyl/guaiacyl (S/G) ratio in straw while the lignin content, straw mechanical properties, plant growth habit, and grain characteristics all remained unaffected. Metabolic profiling revealed significant changes in the abundance of 173 features in the HvF5H1-RNAi lines. The drastic changes in the lignin polymer of transgenic lines highlight the plasticity of barley lignification processes and the associated potential for manipulating and improving lignocellulosic biomass as a feedstock for green technologies. On the other hand, our results highlight some differences between the lignin biosynthetic pathway in barley, a temperate climate grass, and the warm climate grass, rice, and underscore potential diversity in the lignin biosynthetic pathways in grasses.
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DOI：
10.1111/tpj.12510
发表时间：
2014-06
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Marita JM;Hatfield RD;Rancour DM;Frost KE
通讯作者：
Frost KE
影响因子：
7.4
作者：
Lapierre C;Sibout R;Laurans F;Lesage-Descauses MC;Déjardin A;Pilate G
通讯作者：
Pilate G
影响因子：
7.2
作者：
Franke, R;McMichael, CM;Chapple, C
通讯作者：
Chapple, C
影响因子：
13.8
作者：
Daly P;McClellan C;Maluk M;Oakey H;Lapierre C;Waugh R;Stephens J;Marshall D;Barakate A;Tsuji Y;Goeminne G;Vanholme R;Boerjan W;Ralph J;Halpin C
通讯作者：
Halpin C
影响因子：
6.1
作者：
Lu, FC;Ralph, J
通讯作者：
Ralph, J