Heat Shock Inhibits Cytokine-Induced Nitric Oxide Synthase Expression by Rat and Human Islets* * This work was supported by research grants from the NIH (DK-52194) and The Tobacco Research Council.
Heat Shock Inhibits Cytokine-Induced Nitric Oxide Synthase Expression by Rat and Human Islets* * This work was supported by research grants from the NIH (DK-52194) and The Tobacco Research Council.
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热休克抑制大鼠和人类胰岛细胞因子诱导的一氧化氮合酶表达* * 这项工作得到了 NIH (DK-52194) 和烟草研究委员会研究经费的支持。
作者：
A. Scarim;M. Heitmeier;J. A. Corbett
本研究观察了热休克对白细胞介素1受体(IL-1β，IL-1)抑制胰岛代谢功能的影响。用IL-1处理大鼠胰岛18h，可有效抑制葡萄糖刺激的胰岛素分泌。如果胰岛在细胞因子刺激前在42℃下预处理60分钟，则IL-1对胰岛素分泌的抑制作用完全被阻止。热休克还可阻止IL-1对胰岛素瘤RINm5F细胞线粒体乌头酸酶活性的抑制。热休克对胰岛代谢功能的保护作用与抑制IL-1刺激的诱导型一氧化氮合酶(iNOS或NOS II)表达有关。42℃热休克60min的胰岛不能表达iNOS(信使RNA或蛋白质)，也不能产生对IL-1的亚硝酸盐。IL-1诱导的大鼠胰岛诱导型一氧化氮合酶的表达需要激活转录调节因子核因子κB(NF-κB)。热休克通过抑制抑制活性来抑制IL-1诱导的核转录因子κB的核定位。
In this study the effects of heat shock on interleukin-1β (IL-1)-induced inhibition of islet metabolic function were examined. Treatment of rat islets for 18 h with IL-1 results in a potent inhibition of glucose-stimulated insulin secretion. The inhibitory effects of IL-1 on insulin secretion are completely prevented if islets are pretreated for 60 min at 42 C before cytokine stimulation. Heat shock also prevents IL-1-induced inhibition of insulinoma RINm5F cell mitochondrial aconitase activity. The protective effects of heat shock on islet metabolic function are associated with the inhibition of IL-1-stimulated inducible nitric oxide synthase (iNOS or NOS II) expression. Islets heat shocked for 60 min at 42 C fail to express iNOS (messenger RNA or protein) or produce nitrite in response to IL-1. IL-1-induced iNOS expression by rat islets requires activation of the transcriptional regulator nuclear factor κB (NF-κB). Heat shock prevents IL-1- induced NF-κB nuclear localization by inhibiting inhibitory pro...
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影响因子：
4.8
作者：
G. Kwon;J. A. Corbett;Charles P. Rodi;Patrick Sullivan;M. L. McDaniel
通讯作者：
G. Kwon;J. A. Corbett;Charles P. Rodi;Patrick Sullivan;M. L. McDaniel
影响因子：
2.9
作者：
GREEN, LC;WAGNER, DA;TANNENBAUM, SR
通讯作者：
TANNENBAUM, SR
DOI：
10.1073/pnas.90.5.1731
发表时间：
1993-03-01
影响因子：
11.1
作者：
CORBETT, JA;SWEETLAND, MA;MCDANIEL, ML
通讯作者：
MCDANIEL, ML
影响因子：
5
作者：
T. P. Misko;W. M. Moore;T. Kasten;G. Nickols;J. Corbett;R. Tilton;M. Mcdaniel;J. Williamson;M. Currie
通讯作者：
T. P. Misko;W. M. Moore;T. Kasten;G. Nickols;J. Corbett;R. Tilton;M. Mcdaniel;J. Williamson;M. Currie
活性降低的热休克转录因子抑制酵母 HSP70 突变体。
影响因子：
5.3
作者：
Halladay,JT;Craig,EA
通讯作者：
Craig,EA