Repeated confocal imaging of individual dendritic spines in the living hippocampal slice: evidence for changes in length and orientation associated with chemically induced LTP

Repeated confocal imaging of individual dendritic spines in the living hippocampal slice: evidence for changes in length and orientation associated with chemically induced LTP
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活体海马切片中单个树突棘的重复共聚焦成像:与化学诱导的 LTP 相关的长度和方向变化的证据

DOI:
10.1523/jneurosci.15-08-05560.1995
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发表时间:
1995
期刊:
International review of cytology
影响因子:
--
通讯作者:
Monte Carlo Sim
Monte Carlo Sim
中科院分区:
--
文献类型:
--
作者:
T. Hosokawa;D. Rusakov;T. V. P. Bliss;Alan Fine;Monte Carlo Sim

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使用共聚焦显微镜结合微滴应用的DIL,我们已经成像和测量单个树突棘的活海马CA1锥体神经元在急性脑切片,前和约3小时后,诱导的长时程增强的化学手段。统计分析的变化,在个别棘的长度,并与Monte Carlo模拟的结果比较,表明两种形式的结构变化发生在化学诱导的长时程增强:小棘的亚群的增长,和角位移的棘。这些变化可能为长时程增强的表达提供了结构基础。
Using confocal microscopy in conjunction with microdrop application of Dil, we have imaged and measured individual dendritic spines of living hippocampal CA1 pyramidal neurons in acute brain slices, before and approximately 3 hr after induction of long-term potentiation by chemical means. Statistical analysis of changes in the length of individual spines, and comparison with results of Monte Carlo simulations, suggests that two forms of structural change occur in chemically induced long-term potentiation: growth of a subpopulation of small spines, and angular displacement of spines. These changes could provide a structural basis for the expression of long-term potentiation.
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