Detection of Genotype 1a and 1f of Hepatitis E Virus in Patients Treated at Tertiary Care Hospitals in Central India
Detection of Genotype 1a and 1f of Hepatitis E Virus in Patients Treated at Tertiary Care Hospitals in Central India
复制标题
印度中部三级护理医院治疗患者戊型肝炎病毒基因型 1a 和 1f 的检测
作者：
R. Pathak;P. Barde
背景和目标：由戊型肝炎病毒（HEV）引起的急性肝炎是一个重要的全球健康问题，特别是在发展中国家。HEV有1个血清型和8个基因型，进一步分为亚型。尽管印度有散发病例和暴发数据，但印度中部缺乏关于HEV流行病学数据的具体信息。本研究旨在了解印度中部HEV的流行病学和分子特征。方法：在2012年7月至2015年6月为期3年的研究中，使用IgM ELISA诊断疑似HEV病例。为了鉴定基因型，进行nRT PCR，PCR产物测序和分析。使用统计工具分析临床和人口统计学数据，以突出趋势。结果：1,369例疑似病例中，戊型肝炎病毒阳性341例（24.9%）。阳性率在男性（69.2%）和> 15-45岁年龄组（72.5%）中明显较高。HEV病例在夏季达到高峰。在研究期间，在该地区检测到基因型1的1a和1f亚型。结论：戊型肝炎病毒是印度中部病毒性肝炎的主要病原体，成人和男性感染的风险较高。在该地区发现了两种亚型的病毒。持续的血清学监测和分子监测将有助于了解HEV感染的流行病学，缓解疫情并帮助提供治疗。
Background and Aims: An acute hepatitis, caused by hepatitis E virus (HEV), is a significant global health concern, especially in developing countries. HEV has 1 serotype and 8 genotypes, which are further divided into subtypes. Despite the availability of sporadic cases and outbreak data from India, specific information regarding HEV epidemiological data is lacking from central India. This study was conducted to understand epidemiological and molecular features of HEV in central India. Methods: In this 3-year study conducted from July 2012 to June 2015, IgM ELISA was used for the diagnosis of suspected HEV cases. For identifying the genotype, nRT PCR was conducted and the PCR products were sequenced and analysed. Clinical and demographic data were analysed using statistical tools to highlight the trends. Result: Out of 1,369 suspected cases, 341 (24.9%) were positive for HEV. The positivity was significantly higher in males (69.2%) and in the age group of > 15–45 years (72.5%). The HEV cases peaked during the summer. Subtypes 1a and 1f of genotype 1 were detected in the area during the study period. Conclusion: HEV is a major aetiological agent of viral hepatitis in central India with adults and males at higher risk of infection. Two subtypes of the virus were detected in the region. Continuous serological surveillance and molecular monitoring will help to understand the epidemiology of HEV infection, outbreak mitigation and aid in providing treatment.
影响因子：
3.8
作者：
Arankalle, VA;Paranjape, S;Walimbe, AM
通讯作者：
Walimbe, AM