The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids.
The Process of Binding and Releasing of Genetic Material from Lipoplexes Based on Trimeric Surfactants and Phospholipids.
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DOI：
10.3390/ijms22147744
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发表时间：
2021-07-20
影响因子：
5.6
通讯作者：
Kozak M
中科院分区：
文献类型：
--
作者：
Polańska Ż;Pietralik-Molińska Z;Wojciechowska D;Moliński A;Weiss M;Skrzypczak A;Kozak M
用于基因治疗的非病毒载体如脂质复合物具有低毒性、高生物相容性和良好的转染效率的特征。具体而言，基于聚合物表面活性剂和磷脂的脂质复合物具有作为基因载体的巨大潜力，这是由于结合遗传物质的能力增加（正电荷倍增），同时降低不期望的效应（脂质的存在使系统更像天然膜）。本研究旨在测试基于三聚表面活性剂和不同组合物的脂质制剂的lipoplexes结合和释放遗传物质的能力，并通过圆二色谱（CD）光谱和原子力显微镜（AFM）表征形成的复合物。通过MTT细胞活力测定和染料排除试验（台盼蓝）对HeLa细胞测试所研究的脂质复合物的细胞毒性。系统中脂质的存在降低了络合所需的表面活性剂浓度（更高的效率），并降低了脂复合物的细胞毒性。表面活性剂/脂质/DNA复合物比表面活性剂/DNA复合物更稳定。表面活性剂分子诱导的遗传物质的凝聚，但脂质的存在显着加剧了这一过程。基于三聚体表面活性剂和脂质制剂的系统，特别是TRI_N和TRI_IMI系统，可以用作递送载体，并且已经证明对于各种长度的DNA是高效、无毒和通用的。
Nonviral vectors for gene therapy such as lipoplexes are characterized by low toxicity, high biocompatibility, and good transfection efficiency. Specifically, lipoplexes based on polymeric surfactants and phospholipids have great potential as gene carriers due to the increased ability to bind genetic material (multiplied positive electric charge) while lowering undesirable effects (the presence of lipids makes the system more like natural membranes). This study aimed to test the ability to bind and release genetic material by lipoplexes based on trimeric surfactants and lipid formulations of different compositions and to characterize formed complexes by circular dichroism (CD) spectroscopy and atomic force microscopy (AFM). The cytotoxicity of studied lipoplexes was tested on HeLa cells by the MTT cell viability assay and the dye exclusion test (trypan blue). The presence of lipids in the system lowered the surfactant concentration required for complexation (higher efficiency) and reduced the cytotoxicity of lipoplexes. Surfactant/lipids/DNA complexes were more stable than surfactant/DNA complexes. Surfactant molecules induced the genetic material condensation, but the presence of lipids significantly intensified this process. Systems based on trimeric surfactants and lipid formulations, particularly TRI_N and TRI_IMI systems, could be used as delivery carrier, and have proven to be highly effective, nontoxic, and universal for DNA of various lengths.
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影响因子：
3.4
作者：
Koynova, Rumiana;Tarahovsky, Yury S.;MacDonald, Robert C.
通讯作者：
MacDonald, Robert C.
影响因子：
5.6
作者：
Andrzejewska, Weronika;Wilkowska, Michalina;Kozak, Maciej
通讯作者：
Kozak, Maciej
影响因子：
14.9
作者：
Kypr J;Kejnovská I;Renciuk D;Vorlícková M
通讯作者：
Vorlícková M
影响因子：
8
作者：
Bozzuto G;Molinari A
通讯作者：
Molinari A
影响因子：
3.5
作者：
Adamcik, Jozef;Klinov, Dmitry V.;Dietler, Giovanni
通讯作者：
Dietler, Giovanni