Storage globulins pass through the Golgi apparatus and multivesicular bodies in the absence of dense vesicle formation during early stages of cotyledon development in mung bean.
Storage globulins pass through the Golgi apparatus and multivesicular bodies in the absence of dense vesicle formation during early stages of cotyledon development in mung bean.
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DOI：
10.1093/jxb/err366
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发表时间：
2012-02
影响因子：
6.9
通讯作者：
Jiang L
中科院分区：
文献类型：
--
作者：
Wang J;Tse YC;Hinz G;Robinson DG;Jiang L
在种子发育成熟过程中，大量储存蛋白被合成并储存在蛋白质储存液泡中。在发育中的种子中，多种机制被提出负责将储存蛋白转运到psv。本研究建立了一种针对绿豆（Vigna radiata）种子贮藏蛋白8S球蛋白的特异性抗体，并通过免疫金电镜观察其在发育中的绿豆子叶中的沉积。结果表明，在子叶发育的早期阶段，多泡体（MVBs）中存在非聚集的8S球蛋白，而密集泡体（DVs）和多泡体（PSV）都无法识别。然而，在子叶发育的后期阶段，在dv和不同的MVBs中都可以看到凝聚的球蛋白，并具有一种新的分裂形式，内部囊泡被推到该细胞器的一个部门。这些不同的MVBs不再对wortmannin敏感。因此，这项研究表明，在种子发育的早期阶段，MVBs可能在将储存蛋白转运到psv中发挥作用，而不是参与DVs。此外，wortmannin处理显示诱导DVs形成聚集体并与质膜融合。
During seed development and maturation, large amounts of storage proteins are synthesized and deposited in protein storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via immunogold electron microscopy in developing mung bean cotyledons. It is demonstrated that non-aggregated 8S globulins are present in multivesicular bodies (MVBs) in early stages of cotyledon development where neither dense vesicles (DVs) nor a PSV were recognizable. However, at later stages of cotyledon development, condensed globulins were visible in both DVs and distinct MVBs with a novel form of partitioning, with the internal vesicles being pushed to one sector of this organelle. These distinct MVBs were no longer sensitive to wortmannin. This study thus indicates a possible role for MVBs in transporting storage proteins to PSVs during the early stage of seed development prior to the involvement of DVs. In addition, wortmannin treatment is shown to induce DVs to form aggregates and to fuse with the plasma membrane.
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影响因子：
7.8
作者：
MATSUOKA, K;BASSHAM, DC;RAIKHEL, NV;NAKAMURA, K
通讯作者：
NAKAMURA, K
影响因子：
4.9
作者：
Lee, GJ;Sohn, EJ;Hwang, I
通讯作者：
Hwang, I
影响因子：
7.2
作者：
Jolliffe, NA;Brown, JC;Frigerio, L
通讯作者：
Frigerio, L
影响因子：
7.2
作者：
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通讯作者：
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影响因子：
4.3
作者：
KRISHNAN, HB;FRANCESCHI, VR;OKITA, TW
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OKITA, TW