Repeated antigen inhalation-induced reproducible early and late asthma in guinea pigs.
Repeated antigen inhalation-induced reproducible early and late asthma in guinea pigs.
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反复吸入抗原可在豚鼠中诱发可重复的早期和晚期哮喘。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
S. Kohno
中科院分区：
文献类型：
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作者：
T. Nabe;N. Shinoda;M. Yamada;T. Sekioka;Y. Saeki;H. Yamamura;S. Kohno
为了建立豚鼠慢性实验性哮喘模型，每4周吸入一次吸附在细小Al(OH)3上的低剂量卵清蛋白(OA)雾化致敏。在相应的致敏作用后第14天，动物被吸入OA雾化。无论是第一次抗原攻击还是第二次抗原攻击，都能明显地引起早期哮喘反应(EAR)，而几乎没有晚期哮喘反应(LAR)。在第三次挑战中，LAR也出现了一些严重的情况。这些双重反应一直被观察到，直到第10次挑战。另一方面，重复吸入/激发，每两周一次，单独使用相同剂量的OA往往会导致耳朵脱敏。此外，在整个实验过程中几乎没有观察到LAR。在两组中，重复吸入抗原后血清中的γ-1和IgE水平升高，但这些抗体水平与耳或LAR的强度之间没有明显的关系。结果表明，本研究建立的具有可复制的EAR和LAR的哮喘模型对于哮喘的研究和抗哮喘药物的评价是非常有利的。
To develop a model of chronic experimental asthma in guinea pigs, the animal was forced to inhale the mist of a low dose of ovalbumin (OA) adsorbed on fine Al(OH)3 for sensitization once every 4 weeks. The animal was challenged by inhalation with the mist of OA on day 14 after the respective sensitizations. Either the first or the second antigen challenge markedly induced an early asthmatic response (EAR), whereas there was hardly any late asthmatic response (LAR). At the 3rd challenge, LAR also emerged with some severity. These dual responses were consistently observed until the 10th challenge. On the other hand, repeated inhalation/challenge, once every 2 weeks, with OA alone at the same dose tended to lead to the desensitization of the EAR. In addition, LAR was hardly observed throughout the experiments. In both groups, gamma 1 and IgE levels in the serum were elevated by the repetitive antigen inhalations, yet no obvious relationship between these antibody levels and the intensity of either EAR or LAR was recognized. The present results indicate that the asthmatic model with reproducible EAR and LAR developed in this study appears to be very beneficial for the investigation of bronchial asthma and for the assessment of anti-asthma drugs.
内皮素通过多种机制收缩豚鼠气管。
DOI：
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发表时间：
1992
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
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作者：
Ninomiya,H;Uchida,Y;Saotome,M;Nomura,A;Ohse,H;Matsumoto,H;Hirata,F;Hasegawa,S
通讯作者：
Hasegawa,S
DOI：
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发表时间：
1990
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
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作者：
Baumgartner,RA;Wills-Karp,M;Kaufman,MJ;Munakata,M;Hirshman,C
通讯作者：
Hirshman,C
上呼吸道对豚鼠抗原诱导的晚期呼吸道阻塞反应的贡献。
DOI：
10.1164/ajrccm/142.1.138
发表时间：
1990
期刊：
The American review of respiratory disease
影响因子：
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作者：
Johns,K;Sorkness,R;Graziano,F;Castleman,W;LemanskeJr,RF
通讯作者：
LemanskeJr,RF