Functional characterization of the rice UDP-glucose 4-epimerase 1, OsUGE1: a potential role in cell wall carbohydrate partitioning during limiting nitrogen conditions.
Functional characterization of the rice UDP-glucose 4-epimerase 1, OsUGE1: a potential role in cell wall carbohydrate partitioning during limiting nitrogen conditions.
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DOI：
10.1371/journal.pone.0096158
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Rothstein SJ
中科院分区：
文献类型：
--
作者：
Guevara DR;El-Kereamy A;Yaish MW;Mei-Bi Y;Rothstein SJ
生长在矿化氮(N)不足水平下的植物经历了N和C代谢的重新编程，这导致了可溶性和不溶性碳水化合物的显着变化。然而，在植物对氮素限制条件的适应过程中，控制碳水化合物分配的遗传因素的信息相对较少。水稻尿苷-二磷酸-(UDP)-葡萄糖4-表位异构酶基因(OsUGE-1)是N反应基因。我们培育了结构性过表达OsUGE-1基因(OsUGE1-OX1-2)的转基因水稻植株。无论测试的氮素水平如何，转基因水稻株系在营养生长期和成熟期的大小与野生型植株相似。然而，与野生型相比，OsUGE1-OX系在次优氮素水平下保持了18-24%的蔗糖含量和12%-22%的纤维素含量。有趣的是，与野生型植物相比，在低氮条件下，OsUGE1-OX株系半纤维素中的半乳糖和葡萄糖比例较高。OsUGE1-OX系在氮素限制期间细胞壁C分配的改变似乎是由OsUGE1通过抑制纤维素合成相关基因OsSus1、OsCesA4、7和9来介导的。这种关系可能暗示了一个新的UDP-葡萄糖沉积到水稻细胞壁复杂的多糖谱的控制点。然而，OsUGE1与N限制期间细胞壁C-分裂之间的直接关系还需要进一步的研究。
Plants grown under inadequate mineralized nitrogen (N) levels undergo N and carbon (C) metabolic re-programming which leads to significant changes in both soluble and insoluble carbohydrate profiles. However, relatively little information is available on the genetic factors controlling carbohydrate partitioning during adaptation to N-limitation conditions in plants. A gene encoding a uridine-diphospho-(UDP)-glucose 4-epimerase (OsUGE-1) from rice (Oryza sativa) was found to be N-responsive. We developed transgenic rice plants to constitutively over-express the OsUGE-1 gene (OsUGE1-OX1–2). The transgenic rice lines were similar in size to wild-type plants at the vegetative stage and at maturity regardless of the N-level tested. However, OsUGE1-OX lines maintained 18–24% more sucrose and 12–22% less cellulose in shoots compared to wild-type when subjected to sub-optimal N-levels. Interestingly, OsUGE1-OX lines maintained proportionally more galactose and glucose in the hemicellulosic polysaccharide profile of plants compared to wild-type plants when grown under low N. The altered cell wall C-partitioning during N-limitation in the OsUGE1-OX lines appears to be mediated by OsUGE1 via the repression of the cellulose synthesis associated genes, OsSus1, OsCesA4, 7, and 9. This relationship may implicate a novel control point for the deposition of UDP-glucose to the complex polysaccharide profiles of rice cell walls. However, a direct relationship between OsUGE1 and cell wall C-partitioning during N-limitation requires further investigation.
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DOI：
10.1073/pnas.0900188106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Coleman, Heather D.;Yan, Jimmy;Mansfield, Shawn D.
通讯作者：
Mansfield, Shawn D.
影响因子：
5.1
作者：
Lian, XM;Wang, SP;Zhang, QF
通讯作者：
Zhang, QF
DOI：
10.3839/jksabc.2009.056
发表时间：
2009-08-01
影响因子：
--
作者：
Kim, Seong-Kyong;Kim, Dae Hwan;Ahn, Joong-Hoon
通讯作者：
Ahn, Joong-Hoon
影响因子：
7.3
作者：
Paul, MJ;Driscoll, SP
通讯作者：
Driscoll, SP
影响因子：
6.9
作者：
Lawlor, DW
通讯作者：
Lawlor, DW