Clp is a "busy" transcription factor in the bacterial warrior, Lysobacter enzymogenes.
Clp is a "busy" transcription factor in the bacterial warrior, Lysobacter enzymogenes.
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Clp 是细菌战士溶酶杆菌中的一个“忙碌”转录因子
DOI：
10.1016/j.csbj.2021.06.020
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发表时间：
2021
影响因子：
6
通讯作者：
Qian G
中科院分区：
文献类型：
--
作者：
Xu K;Lin L;Shen D;Chou SH;Qian G
溶菌酶原是一种强壮的细菌战士，它使用一种“移动攻击”的抗真菌策略，包括由IV型菌毛和抗真菌武器提供动力的抽搐运动。这种策略是由一个“忙碌”转录因子Clp通过多种先前未被描述的机制控制的，包括以独特的模式与DNA结合，直接相互作用或响应各种小分子化学物质，以及与结构独特的蛋白质特异性相关。环AMP受体蛋白（CRP）是细菌中一组具有良好特征的全局转录因子。众所周知，它们通过结合DNA和/或cAMP（一种称为细菌第二信使的配体）来调节许多细胞过程，以控制靶基因的表达。革兰氏阴性溶菌酶原是一种无鞭毛的土壤传播的植物有益细菌，可以对抗丝状真菌和卵菌。在IV型菌毛（T4P）系统的驱动下，这种细菌移动到附近的病原体，并使用“移动攻击”抗真菌策略，通过热稳定抗真菌因子（HSAF）和丰富的裂解酶杀死它们。这种策略是由一种独特的“忙碌”转录因子Clp控制的，Clp是一种类似crp的蛋白质，通过与细菌的另一种普遍存在的第二信使c-di-GMP结合而失活。在这篇综述中，我们总结了目前关于Clp如何通过一系列以前未被描述的机制启动“移动攻击”策略的进展，包括以独特的模式与DNA结合，直接与各种小分子相互作用或响应，以及与采用独特结构的蛋白质特异性相互作用。总之，这些特征突出了Clp在L.酶原中的多功能作用，这是一种对抗真菌病原体的强大细菌战士。
Lysobacter enzymogenes, a robust bacterial warrior uses a “mobile-attack” antifungal strategy comprised of twitching motility powered by the type IV pilus and antifungal weapons. This strategy is controlled by a “busy” transcription factor Clp via multiple previously uncharacterized mechanisms, including binding with DNA in unique modes, interacting directly or responding to diverse small-molecule chemicals, as well as associating specifically with structurally-distinct proteins. Cyclic AMP receptor protein (CRP) is a well-characterized group of global transcription factors in bacteria. They are known to regulate numerous cellular processes by binding DNA and/or cAMP (a ligand called bacterial second messenger) to control target gene expression. Gram-negative Lysobacter enzymogenes is a soilborne, plant-beneficial bacterium without flagella that can fight against filamentous fungi and oomycete. Driven by the type IV pilus (T4P) system, this bacterium moves to nearby pathogens and uses a “mobile-attack” antifungal strategy to kill them via heat-stable antifungal factor (HSAF) and abundant lyases. This strategy is controlled by a unique “busy” transcription factor Clp, which is a CRP-like protein that is inactivated by binding of c-di-GMP, another ubiquitous second messenger of bacteria. In this review, we summarize the current progress in how Clp initiates a “mobile-attack” strategy through a series of previously uncharacterized mechanisms, including binding to DNA in a unique pattern, directly interacting with or responding to various small molecules, and interacting specifically with proteins adopting distinct structure. Together, these characteristics highlight the multifunctional roles of Clp in L. enzymogenes, a powerful bacterial warrior against fungal pathogens.
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DOI：
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发表时间：
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期刊：
The Journal of biological chemistry
影响因子：
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