Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium.
Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium.
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DOI：
10.1186/gb-2008-9-1-r10
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发表时间：
2008-01-17
期刊：
影响因子：
12.3
通讯作者：
Bucher G
中科院分区：
文献类型：
--
作者：
Tomoyasu Y;Miller SC;Tomita S;Schoppmeier M;Grossmann D;Bucher G
拟谷盗属与C. elegans在显示强的系统性RNAi应答中，但不具有C. elegans型RNAi机制;昆虫系统性RNAi可能使用不同的机制。 RNA干扰（RNAi）是一种高度保守的细胞机制。在一些生物体中，如秀丽隐杆线虫，RNAi反应可以全身性传播。一些昆虫也表现出系统性RNAi反应。然而，领先的昆虫模式生物果蝇并没有表现出强大的系统性RNA干扰反应，因此需要另一种模型系统来研究昆虫系统性RNA干扰的分子机制。我们使用Tribolium作为替代模型系统，其表现出强大的系统性RNAi。我们已经从拟谷盗基因组中鉴定了核心RNAi基因，以及可能参与系统性RNAi的基因。系统发育和功能分析表明，赤拟谷盗的核心组成基因比果蝇多，这可能使其对双链RNA（dsRNA）的反应更敏感。我们还鉴定了三个拟谷盗C. elegans sid-1，其编码可能的dsRNA通道。然而，详细的序列分析表明，这些拟谷盗同源物与另一个拟谷盗同源物具有更多的同一性。elegans基因，tag-130。我们分析了tag-130突变体，发现该基因在C.优雅的同样地，拟谷盗属Tribolium类基因似乎不是系统性RNAi所必需的。这些结果表明，昆虫sid-1样基因具有与dsRNA摄取不同的功能。此外，赤拟谷盗缺乏几个在C.优雅的虽然Tribolium和C.虽然线虫显示出强大的系统性RNAi反应，但我们的全基因组调查揭示了这些生物体的RNAi机制之间的显着差异。因此，昆虫可能使用系统性RNAi应答的替代机制。了解这一过程将有助于使其他昆虫服从系统性RNAi，并可能影响害虫控制方法。
Tribolium resembles C. elegans in showing a robust systemic RNAi response, but does not have C. elegans-type RNAi mechanisms; insect systemic RNAi probably uses a different mechanism. RNA interference (RNAi) is a highly conserved cellular mechanism. In some organisms, such as Caenorhabditis elegans, the RNAi response can be transmitted systemically. Some insects also exhibit a systemic RNAi response. However, Drosophila, the leading insect model organism, does not show a robust systemic RNAi response, necessitating another model system to study the molecular mechanism of systemic RNAi in insects. We used Tribolium, which exhibits robust systemic RNAi, as an alternative model system. We have identified the core RNAi genes, as well as genes potentially involved in systemic RNAi, from the Tribolium genome. Both phylogenetic and functional analyses suggest that Tribolium has a somewhat larger inventory of core component genes than Drosophila, perhaps allowing a more sensitive response to double-stranded RNA (dsRNA). We also identified three Tribolium homologs of C. elegans sid-1, which encodes a possible dsRNA channel. However, detailed sequence analysis has revealed that these Tribolium homologs share more identity with another C. elegans gene, tag-130. We analyzed tag-130 mutants, and found that this gene does not have a function in systemic RNAi in C. elegans. Likewise, the Tribolium sid-like genes do not seem to be required for systemic RNAi. These results suggest that insect sid-1-like genes have a different function than dsRNA uptake. Moreover, Tribolium lacks homologs of several genes important for RNAi in C. elegans. Although both Tribolium and C. elegans show a robust systemic RNAi response, our genome-wide survey reveals significant differences between the RNAi mechanisms of these organisms. Thus, insects may use an alternative mechanism for the systemic RNAi response. Understanding this process would assist with rendering other insects amenable to systemic RNAi, and may influence pest control approaches.
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