Identification of seipin-linked factors that act as determinants of a lipid droplet subpopulation.
Identification of seipin-linked factors that act as determinants of a lipid droplet subpopulation.
复制标题
DOI：
10.1083/jcb.201704122
复制
发表时间：
2018-01-02
期刊：
影响因子：
--
通讯作者：
Bohnert M
中科院分区：
文献类型：
--
作者：
Eisenberg-Bord M;Mari M;Weill U;Rosenfeld-Gur E;Moldavski O;Castro IG;Soni KG;Harpaz N;Levine TP;Futerman AH;Reggiori F;Bankaitis VA;Schuldiner M;Bohnert M
Eisenberg-Bord等人描述了一种具有独特蛋白质组的脂滴（LD）亚群，该蛋白质组邻近核-液泡连接接触位点。他们确定了LD机制，它与脂肪代谢障碍因子seipin合作，作为LD身份的关键决定因素，并提出了功能性细胞器多样化的机制。已经观察到单个细胞的脂滴（LD）池内的功能异质性，但潜在的机制仍然是谜。在这里，我们报告一个专门的LD亚群的特点是一个独特的蛋白质组和一个明确的地理位置在核-空泡交界处接触网站的识别。在寻找决定这些LD身份的因素时，我们筛选了106，000个酵母突变体，以寻找亚群标记Pdr 16的靶向丢失，并将Ldo 45（45 kD的LD组织蛋白）鉴定为关键的靶向决定因素。Ldo 45是连接两个相邻基因（YMR 147 W和YMR 148 W/OSW 5/LDO 16）的剪接事件的产物。我们发现，LDO蛋白与LD生物合成组分seipin合作，并通过定义定位和表面蛋白组成建立LD身份。我们的研究提出了一种建立细胞器功能分化的机制，为更好地理解细胞中的代谢决定打开了大门。
Eisenberg-Bord et al. describe a lipid droplet (LD) subpopulation with a unique proteome, which is adjacent to the nucleus–vacuole junction contact site. They identify the LD machinery, which cooperates with the lipodystrophy factor seipin as a key determinant of LD identity and suggest a mechanism for functional organelle diversification. Functional heterogeneity within the lipid droplet (LD) pool of a single cell has been observed, yet the underlying mechanisms remain enigmatic. Here, we report on identification of a specialized LD subpopulation characterized by a unique proteome and a defined geographical location at the nucleus–vacuole junction contact site. In search for factors determining identity of these LDs, we screened ∼6,000 yeast mutants for loss of targeting of the subpopulation marker Pdr16 and identified Ldo45 (LD organization protein of 45 kD) as a crucial targeting determinant. Ldo45 is the product of a splicing event connecting two adjacent genes (YMR147W and YMR148W/OSW5/LDO16). We show that Ldo proteins cooperate with the LD biogenesis component seipin and establish LD identity by defining positioning and surface-protein composition. Our studies suggest a mechanism to establish functional differentiation of organelles, opening the door to better understanding of metabolic decisions in cells.
登录
查看更多内容
影响因子：
6.5
作者：
Currie E;Guo X;Christiano R;Chitraju C;Kory N;Harrison K;Haas J;Walther TC;Farese RV Jr
通讯作者：
Farese RV Jr
影响因子：
64.5
作者：
Ast, Tslil;Cohen, Galit;Schuldiner, Maya
通讯作者：
Schuldiner, Maya
DOI：
10.1007/978-1-61779-276-2_8
发表时间：
2011-01-01
期刊：
NETWORK BIOLOGY: METHODS AND APPLICATIONS
影响因子：
--
作者：
Cohen, Yifat;Schuldiner, Maya
通讯作者：
Schuldiner, Maya
影响因子：
64.8
作者：
Guo, Yi;Walther, Tobias C.;Farese, Robert V., Jr.
通讯作者：
Farese, Robert V., Jr.
影响因子：
13.6
作者：
Agarwal, AK;Garg, A
通讯作者：
Garg, A