High fat diet induces airway hyperresponsiveness in mice.
High fat diet induces airway hyperresponsiveness in mice.
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DOI：
10.1038/s41598-018-24759-4
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发表时间：
2018-04-23
影响因子：
4.6
通讯作者：
Polotsky VY
中科院分区：
文献类型：
--
作者：
Fricke K;Vieira M;Younas H;Shin MK;Bevans-Fonti S;Berger S;Lee R;D'Alessio FR;Zhong Q;Nelson A;Loube J;Sanchez I;Hansel NN;Mitzner W;Polotsky VY
本实验旨在观察高脂饮食对小鼠气道高反应性的影响。23只成年雄性C57 BL/6 J小鼠用HFD或常规饲料喂养两周。采用强迫振荡法测定基线时和乙酰甲胆碱1、3、10和30 mg/mL气雾剂激发后的总呼吸阻力。进行支气管肺泡灌洗（BAL）。沿着多种细胞因子的基因表达，测定肺组织脂质水平和脂质过氧化反应。消化肺，测定肺巨噬细胞的IL-1β分泌。与普通饲料相比，HFD喂养导致体重增加11%。HFD在基线时不影响呼吸阻力，但与普通饲料相比显著增强了对乙酰甲胆碱的气道反应（在30 mg/ml乙酰甲胆碱时增加40.5 ± 17.7%，p < 0.05）。HFD诱导肺中IL-1β基因表达增加3.2 ± 0.6倍（p < 0.001），IL-1β分泌增加38倍。BAL没有变化，任何其他细胞因子、脂质水平或脂质过氧化反应也没有变化。因此，HFD在小鼠中诱导AHR，然后发生显著肥胖，这与肺IL-1β的上调有关。
The experiment was conducted to examine the effect of a high fat diet (HFD) on airway hyperresponsiveness (AHR) in mice. Twenty-three adult male C57BL/6 J mice were fed with HFD or regular chow diet for two weeks. The total respiratory resistance was measured by forced oscillation technique at baseline and after methacholine aerosol challenge at 1, 3, 10 and 30 mg/mL. Bronchoalveolar lavage (BAL) was performed. Lipid levels and lipid peroxidation in lung tissue were measured along with gene expression of multiple cytokines. Lungs were digested, and IL-1β secretion by pulmonary macrophages was determined. HFD feeding resulted in 11% higher body weight compared to chow. HFD did not affect respiratory resistance at baseline, but significantly augmented airway responses to methacholine compared to chow diet (40.5 ± 17.7% increase at 30 mg/ml methacholine, p < 0.05). HFD induced a 3.2 ± 0.6 fold increase in IL-1β gene expression (p < 0.001) and a 38 fold increase in IL-1β secretion in the lungs. There was no change in BAL and no change in any other cytokines, lipid levels or lipid peroxidation. Hence, HFD induced AHR in mice prior to the development of significant obesity which was associated with up-regulation of pulmonary IL-1β.
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DOI：
10.1073/pnas.96.12.7047
发表时间：
1999-06-08
影响因子：
11.1
作者：
Luheshi, GN;Gardner, JD;Rothwell, NJ
通讯作者：
Rothwell, NJ
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
4.5
作者：
Yang, Wanwei;Cao, Meng;Liu, Chao
通讯作者：
Liu, Chao