Assessment by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long-Term Sample Storage.
Assessment by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long-Term Sample Storage.
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DOI：
10.1002/prca.201700047
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发表时间：
2018-05
期刊：
影响因子：
--
通讯作者：
Nomura F
中科院分区：
文献类型：
--
作者：
Tsuchida S;Satoh M;Umemura H;Sogawa K;Takiwaki M;Ishige T;Miyabayashi Y;Iwasawa Y;Kobayashi S;Beppu M;Nishimura M;Kodera Y;Matsushita K;Nomura F
人类血清和血浆经常被用作蛋白质组学分析的临床标本，而人类血清的肽肽分析是一种很有前途的工具，用于鉴定新的疾病相关生物标志物。基质辅助激光解吸/电离飞行时间质谱法（MALDI - TOF MS）广泛应用于肽组生物标志物的发现。需要仔细的样品收集和处理，因为两者都可能对血清肽球模式产生深远的影响，但分析前变量对血清肽球谱的影响尚未完全阐明。本研究调查了分析前变量，包括储存温度、持续时间（长达12个月）和解冻方法，对基于MALDI - TOF质谱的血清肽丘模式的影响。使用自动ClinProtRobot系统用弱阳离子交换剂磁珠预处理等份血清样品，然后用MALDI - TOF ms分析。根据样品处理变量的不同，观察到峰强度的显著差异。这些峰可以用作样品质量标记，用MALDI - TOF质谱法评估长期储存对血清肽酶谱的影响。
Human serum and plasma are often used as clinical specimens in proteomics analyses, and peptidome profiling of human serum is a promising tool for identifying novel disease‐associated biomarkers. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) is widely used for peptidomic biomarker discovery. Careful sample collection and handling are required as either can have a profound impact on serum peptidome patterns, yet the effects of preanalytical variables on serum peptidome profiles have not been completely elucidated. The present study investigated the effects of preanalytical variables, including storage temperature, duration (up to 12 months), and thawing methods, on MALDI‐TOF MS‐based serum peptidome patterns. Aliquots of serum samples were pretreated with weak cation exchanger magnetic beads using an automated ClinProtRobot system and then analyzed by MALDI‐TOF MS. A number of significant differences in peak intensities were observed depending on sample processing variables. These peaks can be used as sample quality markers to assess the effects of long‐term storage on serum peptidome profiles using MALDI‐TOF MS.
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通过质谱法进行血清/血浆分析和生物标志物鉴定的样品制备。
DOI：
10.1016/j.chroma.2006.11.054
发表时间：
2007-06-15
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Luque-Garcia JL;Neubert TA
通讯作者：
Neubert TA
影响因子：
3.3
作者：
Malm, Johan;Vegvari, Akos;Marko-Varga, Gyorgy
通讯作者：
Marko-Varga, Gyorgy
影响因子：
11.2
作者：
Del Boccio, Piero;Pieragostino, Damiana;Urbani, Andrea
通讯作者：
Urbani, Andrea
影响因子：
3.3
作者：
Mantini, Dante;Petrucci, Francesca;Urbani, Andrea
通讯作者：
Urbani, Andrea
影响因子：
7.7
作者：
Peakman, Tim C.;Elliott, Paul
通讯作者：
Elliott, Paul