Bombyx E75 isoforms display stage- and tissue-specific responses to 20-hydroxyecdysone.
Bombyx E75 isoforms display stage- and tissue-specific responses to 20-hydroxyecdysone.
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Bombyx E75 亚型对 20-羟基蜕皮激素表现出阶段和组织特异性反应
DOI：
10.1038/srep12114
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发表时间：
2015-07-13
影响因子：
4.6
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Li K;Guo E;Hossain MS;Li Q;Cao Y;Tian L;Deng X;Li S
家蚕核受体基因E75由5′端外显子选择性剪接产生，有BmE 75 A、BmE 75 B和BmE 75 C三种mRNA亚型。从5龄幼虫早期到预蛹阶段，BmE 75 A mRNA和蛋白水平在前胸腺显示类似蜕皮激素滴度的发育概况。在脂肪体，mRNA水平，但不是蛋白水平的所有三个BmE 75亚型与蜕皮激素滴度;此外，蛋白质的所有三个BmE 75亚型消失在prepupal阶段，并修改BmE 75蛋白质具有较小的分子量和细胞质定位发生。用20-羟基蜕皮酮（20-hydroxyecdysone，20 E）和放线菌酮（cycloheximide，CHX）处理5龄幼虫的前胸腺和脂肪体，发现BmE 75 A在mRNA和蛋白水平上对20 E具有初级反应，而BmE 75 B和BmE 75 C对20 E具有不同的反应。在早期游走阶段，RNAi介导的20 E核受体复合物（EcR-USP）基因表达的减少显著降低了两种组织中所有三种BmE 75亚型的mRNA和蛋白水平。总之，BmE 75亚型显示阶段和组织特异性反应20 E在mRNA和蛋白质水平上，而且，它们在蛋白质水平上受到其他未知因素的调控。
Resulted from alternative splicing of the 5′ exons, the nuclear receptor gene E75 in the silkworm, Bombyx mori, processes three mRNA isoforms, BmE75A, BmE75B and BmE75C. From the early 5th larval instar to the prepupal stages, BmE75A mRNA and protein levels in the prothoracic glands display developmental profiles similar to ecdysteroid titer. In the fat body, mRNA levels but not protein levels of all three BmE75 isoforms correlate with ecdysteroid titer; moreover, proteins of all three BmE75 isoforms disappear at the prepupal stages, and a modified BmE75 protein with smaller molecular weight and cytoplasm localization occurs. At the early 5th larval instar stage, treatment of the prothoracic glands and fat body with 20-hydroxyecdysone (20E) and/or cycloheximide (CHX) revealed that BmE75A is 20E primary-responsive at both mRNA and protein levels, while BmE75B and BmE75C exhibit various responses to 20E. At the early wandering stage, RNAi-mediated reduction of gene expression of the 20E nuclear receptor complex, EcR-USP, significantly decreased mRNA and protein levels of all three BmE75 isoforms in both tissues. In conclusion, BmE75 isoforms display stage- and tissue-specific responses to 20E at both mRNA and protein levels; moreover, they are regulated by other unknown factors at the protein level.
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影响因子：
11.4
作者：
KARIM, FD;THUMMEL, CS
通讯作者：
THUMMEL, CS
影响因子：
3.8
作者：
Liu, Hanhan;Jia, Qiangqiang;Li, Sheng
通讯作者：
Li, Sheng
影响因子：
2.9
作者：
Aicart-Ramos C;Valhondo Falcón M;Ortiz de Montellano PR;Rodriguez-Crespo I
通讯作者：
Rodriguez-Crespo I
DOI：
10.1534/g3.113.006239
发表时间：
2013-09-04
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Suetsugu Y;Futahashi R;Kanamori H;Kadono-Okuda K;Sasanuma S;Narukawa J;Ajimura M;Jouraku A;Namiki N;Shimomura M;Sezutsu H;Osanai-Futahashi M;Suzuki MG;Daimon T;Shinoda T;Taniai K;Asaoka K;Niwa R;Kawaoka S;Katsuma S;Tamura T;Noda H;Kasahara M;Sugano S;Suzuki Y;Fujiwara H;Kataoka H;Arunkumar KP;Tomar A;Nagaraju J;Goldsmith MR;Feng Q;Xia Q;Yamamoto K;Shimada T;Mita K
通讯作者：
Mita K
MET 是家蚕变态过程中 20-羟基蜕皮激素发挥最大作用所必需的
影响因子：
3.7
作者：
Guo E;He Q;Liu S;Tian L;Sheng Z;Peng Q;Guan J;Shi M;Li K;Gilbert LI;Wang J;Cao Y;Li S
通讯作者：
Li S