Eco RI* specificity and hydrogen bonding.
Eco RI* specificity and hydrogen bonding.
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Eco RI* 特异性和氢键。
DOI：
10.1089/dna.1.1982.1.117
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
P. Greene
中科院分区：
文献类型：
--
作者：
J. Rosenberg;P. Greene
在标准条件下，Eco RI内切酶唯一识别反向重复的GAATTC。然而，这种特异性在非标准条件下被分解为已被称为Eco RI*特异性，其中许多其他序列被识别。我们在这里表明，所有已知的Eco RI*位的水解率可以用层次来概括：第一位的G远大于A大于T，第二位和第三位的A远大于[G，C]远大于T，以及最后三位的相应补体。这与识别模型是一致的，该模型假设在标准条件下每个碱基对有两个特定的氢键。在Eco RI*条件下，用水随机替换其中的一个或多个，并且序列在适当层次中的位置主要由保留的氢键的数量决定。这些保留的氢键是常见的识别特征，可以通过检查DNA来识别。由此确定的Eco RI的识别点都落在DNA的主沟内。
Under standard conditions, Eco RI endonuclease uniquely recognizes the inverted repeat GAATTC. However, this specificity breaks down under non-standard conditions into what has been termed Eco RI* specificity, wherein many other sequences are recognized. We show here that the hydrolysis rates at all known Eco RI* sites can be summarized by the hierarchies: G much greater than A greater than T much greater than C at the first position, A much greater than [G,C] much greater than T at the second and third position, and the corresponding complements at the last three positions. This is consistent with a recognition model which assumes that there are two specific hydrogen bonds per base pair under standard conditions. One or more of these are randomly replaced by water under Eco RI* conditions and the position of a sequence within the appropriate hierarchy is primarily determined by the number of retained hydrogen bonds. These retained hydrogen bonds are common recognition features that can be identified by examining the DNA. The recognition points thereby identified for Eco RI all fall within the major groove of the DNA.
DNA 位点识别和 Eco RI 核酸内切酶特异性降低。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
WoodburyJr,CP;Hagenbüchle,O;vonHippel,PH
通讯作者：
vonHippel,PH
EcoRI 限制性内切酶-DNA 复合物的初步 X 射线衍射研究。
影响因子：
5.6
作者：
Young,T;Kim,S;Modrich,P;Beth,A;Jay,E
通讯作者：
Jay,E