Perivascular mural cells of the mouse choroid demonstrate morphological diversity that is correlated to vasoregulatory function.
Perivascular mural cells of the mouse choroid demonstrate morphological diversity that is correlated to vasoregulatory function.
复制标题
DOI：
10.1371/journal.pone.0053386
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wong WT
中科院分区：
文献类型：
--
作者：
Condren AB;Kumar A;Mettu P;Liang KJ;Zhao L;Tsai JY;Fariss RN;Wong WT
脉络膜的血管周壁细胞与生理功能以及视网膜疾病的发病机制有关。然而，关于它们的形式和功能的细节还不清楚。我们的目的是表征脉络膜壁细胞在成年小鼠脉络膜的分布和形态，并将这些与它们的收缩行为。从α-平滑肌肌动蛋白（α-SMA）启动子驱动绿色荧光蛋白（GFP）表达的白化病转基因小鼠中制备巩膜脉络膜平板外植体。因此，使活脉络膜中表达α-SMA的平滑肌细胞和周细胞发出荧光，并使用共聚焦显微镜和活细胞原位成像进行成像。 脉络膜血管周壁细胞在不同层次的血管系统中的分布和形态表现出显着的多样性。它们的范围从在主动脉中提供完整血管覆盖的密集包装的周向定向细胞到稀疏分布在终末小动脉上的宽间隔星状细胞。每个水平的壁细胞对收缩蛋白α-SMA和结蛋白呈免疫阳性，并表现出对内皮素-1和钙离子载体A23187的反应性血管收缩运动，以及对钙螯合剂BAPTA的反应性血管舒张。血管调节性收缩反应的显著性随壁细胞形态和密度而变化，并且在具有周向细胞形态的壁细胞密集覆盖的血管中更大。在脉络膜毛细血管中，周细胞呈稀疏的水平分布，并且仅选择性地分布于脉络膜毛细血管的巩膜表面。血管周围壁细胞的多样性和区域特化可能有助于脉络膜血管调节的不同要求。α-SMA-GFP转基因白化病小鼠模型为脉络膜壁细胞的形态学和功能研究提供了一个有用的、完整的系统。
Perivascular mural cells of the choroid have been implicated in physiological functioning as well as in retinal disease pathogenesis. However details regarding their form and function are not well understood. We aim to characterize choroidal mural cells in the adult mouse choroid in terms of their distribution and morphology, and correlate these to their contractile behavior. Sclerochoroidal flat-mounted explants were prepared from albino transgenic mice in which the α-smooth muscle actin (α-SMA) promoter drives the expression of green fluorescent protein (GFP). α-SMA-expressing smooth muscle cells and pericytes in the living choroid were thereby rendered fluorescent and imaged with confocal microscopy and live-cell imaging in situ. Choroidal perivascular mural cells demonstrate significant diversity in terms of their distribution and morphology at different levels of the vasculature. They range from densely-packed circumferentially-oriented cells that provide complete vascular coverage in primary arteries to widely-spaced stellate-shaped cells that are distributed sparsely over terminal arterioles. Mural cells at each level are immunopositive for contractile proteins α-SMA and desmin and demonstrate vasoconstrictory contractile movements in response to endothelin-1 and the calcium ionophore, A23187, and vasodilation in response to the calcium chelator, BAPTA. The prominence of vasoregulatory contractile responses varies with mural cell morphology and density, and is greater in vessels with dense coverage of mural cells with circumferential cellular morphologies. In the choriocapillaris, pericytes demonstrate a sparse, horizontal distribution and are selectively distributed only to the scleral surface of the choriocapillaris. Diversity and regional specialization of perivascular mural cells may subserve varying requirements for vasoregulation in the choroid. The model of the α-SMA-GFP transgenic albino mouse provides a useful and intact system for the morphological and functional study of choroidal mural cells.
登录
查看更多内容
影响因子：
17.8
作者：
Kur, Joanna;Newman, Eric A.;Chan-Ling, Tailoi
通讯作者：
Chan-Ling, Tailoi
影响因子：
3.7
作者：
Mata, Nathan L.;Vogel, Roger
通讯作者：
Vogel, Roger
影响因子：
4.4
作者：
Boltz, Agnes;Luksch, Alexandra;Schmetterer, Leopold
通讯作者：
Schmetterer, Leopold
影响因子：
6
作者：
Jo, Nobuo;Mailhos, Carolina;Shima, David T.
通讯作者：
Shima, David T.
影响因子：
4.4
作者：
Chan-Ling, Tailoi;Koina, Mark E.;Baxter, Louise
通讯作者：
Baxter, Louise