Localization of muscarinic receptor subtype mRNAs in human lung.
Localization of muscarinic receptor subtype mRNAs in human lung.
复制标题
毒蕈碱受体亚型 mRNA 在人肺中的定位。
DOI：
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发表时间：
1992
影响因子：
6.4
通讯作者：
P. Barnes
中科院分区：
文献类型：
--
作者：
Judith C.W. Mak;James N. Baraniuk;P. Barnes
气道平滑肌中m2 mRNA;气道上皮、气道平滑肌和粘膜下腺体中m3 mRNA。在任何细胞结构中均未检测到m4和m5 mRNA。通过北方印迹分析证实了各种毒蕈碱受体亚型mRNA的存在。只有人肺mRNA杂交的M1探针，得到一个单一的3.2 kb的转录。培养的人气道平滑肌细胞的mRNA分别产生约6.0kb和4.5kb的m2和m3杂交带，而培养的气道上皮细胞的mRNA仅产生4.5kb的m3杂交带。除了气道上皮，有一个很好的相关性之间的分布mRNA的原位杂交和受体亚型的分布放射自显影映射。这些结果可能具有重要的临床意义，也引起了进一步的研究肺毒蕈碱受体亚型在健康和疾病的基因调控。
m2 mRNA in airway smooth muscle; m3 mRNA in airway epithelium, airway smooth muscle, and submucosal glands. No detection of m4 and m5 mRNAs was observed in any cellular structures. The presence of various muscarinic receptor subtype mRNAs was confirmed by Northern blot analysis. Only human lung mRNA hybridized to the m1 probe giving a single 3.2 kb transcript. mRNA from the human cultured airway smooth muscle cells gave m2 and m3 hybridization bands of about 6.0 kb and 4.5 kb, respectively, while mRNA from the cultured airway epithelial cells gave only m3 hybridization band of 4.5 kb. With the exception of the airway epithelium, there was a good correlation between the distribution of mRNAs by in situ hybridization and the distribution of receptor subtypes by autoradiographic mapping. These results may have an important clinical implication, and also give rise to further investigation of gene regulation of pulmonary muscarinic receptor subtypes in health and disease.
人外周肺毒蕈碱胆碱能受体亚型的表征。
DOI：
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发表时间：
1988
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Bloom,JW;Halonen,M;Yamamura,HI
通讯作者：
Yamamura,HI
影响因子：
3.6
作者：
F. Dörje;A. Levey;M. Brann
通讯作者：
F. Dörje;A. Levey;M. Brann
培养中的人气道平滑肌。
影响因子：
2.6
作者：
Twort,C;VanBreemen,C
通讯作者：
VanBreemen,C