Molecular structural heterogeneity of bisphenols governs their serum albumin binding
Molecular structural heterogeneity of bisphenols governs their serum albumin binding
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双酚的分子结构异质性控制其血清白蛋白结合
DOI：
10.1016/j.scitotenv.2021.146499
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发表时间：
2021-08
影响因子：
9.8
通讯作者：
Guibin Jiang
中科院分区：
文献类型：
--
作者：
Xingchen Zhao;Peng-hui Li;Shanjun Song;Huiyu Wang;Lining Zhao;Wansong Zong;Haiyan Zhang;Guangbo Qu;Ligang Hu;Zongwei Cai;Guibin Jiang
双酚A及其类似物(双酚F，BPF；双酚AF，BPAF；双酚S，BPS；四溴双酚A，TbBPA)通过血液转运，并与靶器官或细胞的雌激素受体结合。平衡透析法测得的结合常数分别为5.14×103M−1、1.05×104M−1和7.89×103M−1，结合能力中等，结合部位多。HSA-水分配系数(logKHW>；3)大于其辛醇-水分配系数，其大小顺序为：TBBPA>；BPAF>；BPA(3.75)>；BPF(3.61)>；BPS(3.27)。Trp214在人血清白蛋白(HSA)中的荧光猝灭是由双酚的官能团和取代基决定的。结果如下：tBPA(4.41×1014M−1S−1)；bps(4.08×1012M−1S−1)>；bPAF(1.20×1012M−1S−1)>；bpF(3.06×1011M−1S−1)≈bPA(4.47×1011M−1S−1)，与分子对接结果一致。在此过程中，HSA的酶学性质也发生了变化，除BPS外，KmandVmax降低，催化效率提高，这可能有助于其他药物的水解性。然而，由于可逆结合，蛋白质的天然构象发生了局部适应性变化。总体而言，这些数据为BP在人体血液中的运输提供了一个机制解释，这可能会影响它们的滞留和毒性。
Bisphenol A (BPA) and its analogs (bisphenol F, BPF; bisphenol AF, BPAF; bisphenol S, BPS; and tetrabromobisphenol A, TBBPA) are transported by blood and bind estrogen receptors of target organs or cells. They were confirmed to bind human serum albumin (HSA) in blood, and the binding constants of BPA (5.14 × 103M−1), BPF (1.05 × 104M−1), and BPS (7.89 × 103M−1) determined via equilibrium dialysis shows moderate binding ability with multiple binding sites. The HSA-water partition coefficients (logKHW> 3) are greater than their octanol-water distribution coefficients, and may follow the order: TBBPA > BPAF > BPA (3.75) > BPF (3.61) > BPS (3.27). Functional groups and substitutions of bisphenols (BPs) determine the fluorescence quenching of Trp214 in HSA. The effects follow: TBBPA (4.41 × 1014M−1s−1) » BPS (4.08 × 1012M−1s−1) > BPAF (1.20 × 1012M−1s−1) > BPF (3.06 × 1011M−1s−1) ≈ BPA (4.47 × 1011M−1s−1), which is in line with the molecular docking results. In this process, the enzymatic characteristics of HSA were changed simultaneously, as evidenced by decreasedKmandVmaxexcept for BPS (increasedKmandVmax) and increased catalytic efficiency, which may improve the hydrolysis of other drugs. However, the native conformation of the protein underwent locally adaptive changes due to the reversible binding. Overall, these data provide a mechanistic explanation for the transport of BPs in human blood, which may affect their retention and toxicity.
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影响因子：
8.8
作者：
Xiao-yun Xie;Xiao-ru Wang;Xiangmei Xu;Huijun Sun;Xingguo Chen
通讯作者：
Xiao-yun Xie;Xiao-ru Wang;Xiangmei Xu;Huijun Sun;Xingguo Chen
DOI：
10.1016/j.jphotobiol.2016.08.011
发表时间：
2016-10
期刊：
Journal of photochemistry and photobiology. B, Biology
影响因子：
--
作者：
Rui Zhang;Lining Zhao;Rutao Liu
通讯作者：
Rui Zhang;Lining Zhao;Rutao Liu
影响因子：
4.1
作者：
Sakurama K;Kawai A;Tuan Giam Chuang V;Kanamori Y;Osa M;Taguchi K;Seo H;Maruyama T;Imoto S;Yamasaki K;Otagiri M
通讯作者：
Otagiri M
影响因子：
4.1
作者：
Debruyn, Adrian M. H.;Gobas, Frank A. Pc.
通讯作者：
Gobas, Frank A. Pc.
金纳米棒对红细胞的血液学影响：溶血和血红蛋白构象和功能的变化
DOI：
10.1002/advs.201700296
发表时间：
2017-12
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Zhao X;Lu D;Liu QS;Li Y;Feng R;Hao F;Qu G;Zhou Q;Jiang G
通讯作者：
Jiang G