Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes.
Hematological Effects of Gold Nanorods on Erythrocytes: Hemolysis and Hemoglobin Conformational and Functional Changes.
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金纳米棒对红细胞的血液学影响：溶血和血红蛋白构象和功能的变化
DOI：
10.1002/advs.201700296
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Jiang G
中科院分区：
文献类型：
--
作者：
Zhao X;Lu D;Liu QS;Li Y;Feng R;Hao F;Qu G;Zhou Q;Jiang G
金纳米棒（GNR）是一类独特的金属纳米结构，在生物医学应用中具有诱人的潜力，对其生物安全性的关注也随之增加。血液相容性极其重要，因为它们在体内递送期间不可避免地与血液循环接触。在此，使用两种涂有十六烷基三甲基溴化铵（C-GNR）或聚（对苯乙烯磺酸钠）的GNR来测试它们在血液中的潜在毒理学作用。表面带正电荷的 C-GNR 在遇到红细胞时可有效诱导溶血。发现了 C-GNR 的细胞内化，它们随后与血红蛋白结合，形成生物缀合物。血红蛋白和 C-GNR（化学计量比 32.7:1）之间的相互作用受静电力调节。色氨酸 (Trp) 等发色团被发现与 C-GNR 相互作用，导致荧光强度增强。蛋白质构象部分改变，表现为α螺旋减少、β折叠增加和血红蛋白无规卷曲。尽管 C-GNR 本质上不会降低血红蛋白的氧结合能力，但它们会阻碍蛋白质释放氧。血红素（氧结合单元）在 C-GNR 处理后从血红蛋白中释放，这可能导致 C-GNR 诱导的溶血。这项研究证明了 GNR 的血液学效应，揭示了它们在生物医学应用中的潜在风险。
Gold nanorods (GNRs) are a unique class of metal nanostructures that have attractive potentials in biomedical applications, and the concern on their biological safety is concomitantly increasing. Hemocompatibility is extremely important as their contact with blood circulation is unavoidable during in vivo delivery. Herein, two kinds of GNRs coated with hexadecyltrimethylammonium bromide (C‐GNRs) or poly(sodium‐p‐styrenesulfonate) are used to test their potential toxicological effects in blood. C‐GNRs with positive surface charges efficiently induce hemolysis when encountering erythrocytes. Cellular internalization of C‐GNRs is found, and they subsequently bind with hemoglobin, forming bioconjugates. The interaction between hemoglobin and C‐GNR (stoichiometry 32.7:1) is regulated by electrostatic forces. Chromophores like tryptophan (Trp) are found to interact with C‐GNRs, causing enhancement in fluorescence intensity. The conformation of protein is partially altered, evidenced by decrease in α‐helical, increase in β‐sheet and random coil of hemoglobin. Although C‐GNRs do not essentially decrease oxygen binding capacity of hemoglobin, they hamper oxygen release from the protein. Heme, the oxygen binding unit, releases from hemoglobin upon C‐GNR treatment, which could contribute to C‐GNR‐induced hemolysis. This study demonstrates the hematological effects of GNRs, revealing their potential risk in biomedical applications.
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影响因子：
17.1
作者：
Leonov, Alexei P.;Zheng, Jiwen;Clogston, Jeffrey D.;Stern, Stephan T.;Patri, Anil K.;Wei, Alexander
通讯作者：
Wei, Alexander
影响因子：
17.1
作者：
Lundqvist, Martin;Stigler, Johannes;Dawson, Kenneth
通讯作者：
Dawson, Kenneth
影响因子：
4
作者：
Kim, Kang;Huang, Sheng-Wen;Kaplan, Mariana J.
通讯作者：
Kaplan, Mariana J.
影响因子：
5
作者：
Lau, Irene P.;Chen, Huanjun;Kong, Siu K.
通讯作者：
Kong, Siu K.
影响因子：
3.3
作者：
Jain, PK;Lee, KS;El-Sayed, MA
通讯作者：
El-Sayed, MA