Electrothermal supercharging in mass spectrometry and tandem mass spectrometry of native proteins.
Electrothermal supercharging in mass spectrometry and tandem mass spectrometry of native proteins.
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DOI：
10.1021/ac302256d
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发表时间：
2013-01-02
影响因子：
7.4
通讯作者：
Williams, Evan R.
中科院分区：
文献类型：
--
作者：
Cassou, Catherine A.;Sterling, Harry J.;Susa, Anna C.;Williams, Evan R.
通过电喷雾电离从缓冲水溶液中形成的蛋白质离子的电热增压，与从相同的溶液中形成离子但具有较低的喷雾电位的天然质谱法相比，导致最大和平均电荷状态的显着增加。对于所研究的9种蛋白质中的8种，由电热增压的天然溶液形成的质子化离子的最大电荷态大于由水/甲醇/酸组成的常规变性溶液所获得的质子化离子，尽管由于更折叠的低电荷态结构的小群体的贡献，平均电荷略低。在这些条件下，电热增压对阴离子的效果略低于对阳离子的效果。同样的细胞色素c的高电荷态离子由原生溶液和变性溶液的电热增压形成，通过电子转移解离，获得了等效的序列覆盖率（80%）。电热增压有利于将天然环境中蛋白质的结构研究与具有有限高m/z能力的质谱结合起来，并显著提高从具有天然结构和活性的分子溶液中形成的蛋白质离子的串联质谱性能。
Electrothermal supercharging of protein ions formed by electrospray ionization from buffered aqueous solutions results in significant increases to both the maximum and average charge states compared to native mass spectrometry in which ions are formed from the same solutions but with lower spray potentials. For eight of the nine proteins investigated, the maximum charge states of protonated ions formed from native solutions with electrothermal supercharging is greater than those obtained from conventional denaturing solutions consisting of water/methanol/acid, although the average charging is slightly lower owing to contributions of small populations of more folded low charge-state structures. Under these conditions, electrothermal supercharging is slightly less effective for anions than for cations. Equivalent sequence coverage (80%) is obtained with electron transfer dissociation of the same high charge-state ion of cytochrome c formed by electrothermal supercharging from native solutions and from denaturing solutions. Electrothermal supercharging should be advantageous for combining structural studies of proteins in native environments with mass spectrometers that have limited high m/z capabilities and for significantly improving tandem mass spectrometry performance for protein ions formed from solutions in which the molecules have native structures and activities.
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DOI：
10.1007/s13361-012-0393-z
发表时间：
2012-07-01
影响因子：
3.2
作者：
Hall, Zoe;Robinson, Carol V.
通讯作者：
Robinson, Carol V.
影响因子：
15
作者：
Breuker, K;Oh, HB;McLafferty, FW
通讯作者：
McLafferty, FW
DOI：
10.1073/pnas.93.7.3143
发表时间：
1996-04-02
影响因子：
11.1
作者：
Gross, DS;Schnier, PD;Williams, ER
通讯作者：
Williams, ER
影响因子：
56.9
作者：
Berkenkamp, S;Kirpekar, F;Hillenkamp, F
通讯作者：
Hillenkamp, F
影响因子：
16.6
作者：
Breuker, K;McLafferty, FW
通讯作者：
McLafferty, FW