Control of rat muscle nitrate levels after perturbation of steady state dietary nitrate intake.
Control of rat muscle nitrate levels after perturbation of steady state dietary nitrate intake.
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DOI：
10.1016/j.niox.2021.03.003
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发表时间：
2021-05-01
期刊：
影响因子：
--
通讯作者：
Piknova B
中科院分区：
文献类型：
--
作者：
Park JW;Thomas SM;Schechter AN;Piknova B
在哺乳动物体内，硝酸盐和亚硝酸盐离子作为一氧化氮（NO）来源，补充一氧化氮酶的作用是近年来研究的热点。我们之前报道过大鼠骨骼肌作为硝酸盐储存库，其储存的硝酸盐数量高度依赖于膳食硝酸盐的有效性，以及NOS1酶的合成和随后的利用。我们发现，在NO需求增加的条件下，这个硝酸盐库被原位用于生成亚硝酸盐和NO，至少部分是通过黄嘌呤氧化还原酶（XOR）的硝酸盐还原酶活性。我们现在进一步研究在首先增加饮用水中的硝酸盐水平然后又恢复到原来的摄入量水平后，大鼠骨骼肌中硝酸盐/亚硝酸盐通量的动力学。分析了肝脏、血液和几个骨骼肌样本中的硝酸盐/亚硝酸盐水平，并测量了参与硝酸盐代谢和运输的蛋白质的表达。硝酸盐供应增加，所有测量组织中硝酸盐和亚硝酸盐水平升高。令人惊讶的是，在终止高硝酸盐饮食后，肝脏和所有肌肉样本中的两种离子水平首先下降到低于原始基线的水平。在整个实验过程中，肌肉组织中的XOR表达逐渐增加，这可能导致硝酸盐向亚硝酸盐的还原增强。我们还注意到不同类型肌肉中硝酸盐基础水平的差异。这些发现表明肌肉硝酸盐水平的复杂控制，可能通过多种过程来保持其细胞内水平。
The roles of nitrate and nitrite ions as nitric oxide (NO) sources in mammals, complementing NOS enzymes, have recently been the focus of much research. We previously reported that rat skeletal muscle serves as a nitrate reservoir, with the amount of stored nitrate being highly dependent on dietary nitrate availability, as well as its synthesis by NOS1 enzymes and its subsequent utilization. We showed that at conditions of increased NO need, this nitrate reservoir is used in situ to generate nitrite and NO, at least in part via the nitrate reductase activity of xanthine oxidoreductase (XOR). We now further investigate the dynamics of nitrate/nitrite fluxes in rat skeletal muscle after first increasing nitrate levels in drinking water and then returning to the original intake level. Nitrate/nitrite levels were analyzed in liver, blood and several skeletal muscle samples, and expression of proteins involved in nitrate metabolism and transport were also measured. Increased nitrate supply elevated nitrate and nitrite levels in all measured tissues. Surprisingly, after high nitrate diet termination, levels of both ions in liver and all muscle samples first declined to lower levels than the original baseline. During the course of the overall experiment there was a gradual increase of XOR expression in muscle tissue, which likely led to enhanced nitrate to nitrite reduction. We also noted differences in basal levels of nitrate in the different types of muscles. These findings suggest complex control of muscle nitrate levels, perhaps with multiple processes to preserve its intracellular levels.
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DOI：
10.1073/pnas.97.21.11482
发表时间：
2000-10-10
影响因子：
11.1
作者：
Gladwin, MT;Shelhamer, JH;Cannon, RO
通讯作者：
Cannon, RO
影响因子：
3.9
作者：
Gilliard, Cameron N.;Lam, Jeff K.;Piknova, Barbora
通讯作者：
Piknova, Barbora
影响因子：
20.3
作者：
Crawford, JH;Isbell, TS;Patel, RP
通讯作者：
Patel, RP
影响因子：
3.9
作者：
Jose Poderoso, Juan;Helfenberger, Katia;Poderoso, Cecilia
通讯作者：
Poderoso, Cecilia
影响因子：
2.9
作者：
Li, HT;Samouilov, A;Zweier, JL
通讯作者：
Zweier, JL