Sperm preservation by freeze-drying for the conservation of wild animals.
Sperm preservation by freeze-drying for the conservation of wild animals.
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DOI：
10.1371/journal.pone.0113381
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Inoue-Murayama M
中科院分区：
文献类型：
--
作者：
Kaneko T;Ito H;Sakamoto H;Onuma M;Inoue-Murayama M
精子保存是实验动物、家畜和野生动物生物资源保存的一项有用技术。一种新的保存方法已经开发出来，可以使精子在4 ℃的冰箱中长时间保存。将精子在含有10 mM Tris和1 mM EDTA的溶液中冷冻干燥。使用这种方法，精子的储存和运输不需要液氮。我们证明，黑猩猩，长颈鹿，美洲虎，黄鼠狼和长毛大鼠的精子冷冻干燥后仍然有活力。在所有物种中，将冷冻干燥的精子注射到小鼠卵母细胞中后均形成原核。这些结果虽然是初步的，但对未来建立“冻干动物园”以保护野生动物有一定的参考价值。
Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4°C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of “freeze-drying zoo” to conserve wild animals.
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影响因子：
2.7
作者：
Kaneko, Takehito;Kimura, Shinya;Nakagata, Naomi
通讯作者：
Nakagata, Naomi
影响因子：
3.6
作者：
Kwon, IK;Park, KE;Niwa, K
通讯作者：
Niwa, K
影响因子：
2.8
作者：
Kaneko, T.;Kimura, S.;Nakagata, N.
通讯作者：
Nakagata, N.
DOI：
10.1017/s0967199409005292
发表时间：
2009-08
期刊：
Zygote (Cambridge, England)
影响因子：
--
作者：
Li MW;Willis BJ;Griffey SM;Spearow JL;Lloyd KC
通讯作者：
Lloyd KC
影响因子：
3.6
作者：
Keskintepe, L;Pacholczyk, G;Brackett, BG
通讯作者：
Brackett, BG