Evidence for compensatory upregulation of expressed X-linked genes in mammals, Caenorhabditis elegans and Drosophila melanogaster.
Evidence for compensatory upregulation of expressed X-linked genes in mammals, Caenorhabditis elegans and Drosophila melanogaster.
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许多动物物种使用基于染色体的性别决定机制，这导致了剂量补偿系统的协调进化。剂量补偿不仅纠正了两性之间X染色体数目的不平衡，而且被认为是通过上调X连锁基因的两倍来纠正细胞内由于单等位X连锁表达和双等位常染色体表达引起的剂量失衡(称为Ohno假说)。虽然这一假说得到了单个X连锁基因的表达分析和基于微阵列的转录组分析的很好支持，但最近一项使用RNA测序和蛋白质组学的研究对这一假说提出了挑战。我们获得了新的、独立的RNA序列数据，测量了RNA聚合酶的分布，并重新分析了已发表的哺乳动物、线虫和果蝇的表达数据。我们的分析考虑了X染色体的偏斜基因含量，支持了X连锁基因表达上调以平衡基因组表达的假说。
Many animal species use a chromosome-based mechanism of sex determination, which has led to the coordinate evolution of dosage-compensation systems. Dosage compensation not only corrects the imbalance in the number of X chromosomes between the sexes but also is hypothesized to correct dosage imbalance within cells that is due to monoallelic X-linked expression and biallelic autosomal expression, by upregulating X-linked genes twofold (termed ‘Ohno’s hypothesis’). Although this hypothesis is well supported by expression analyses of individual X-linked genes and by microarray-based transcriptome analyses, it was challenged by a recent study using RNA sequencing and proteomics. We obtained new, independent RNA-seq data, measured RNA polymerase distribution and reanalyzed published expression data in mammals, C. elegans and Drosophila. Our analyses, which take into account the skewed gene content of the X chromosome, support the hypothesis of upregulation of expressed X-linked genes to balance expression of the genome.
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影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
通讯作者：
Allis CD
影响因子：
10.5
作者：
Hamada, FN;Park, PJ;Kuroda, MI
通讯作者：
Kuroda, MI
DOI：
10.1073/pnas.94.17.9244
发表时间：
1997-08-19
影响因子：
11.1
作者：
Adler, DA;Rugarli, EI;Disteche, CM
通讯作者：
Disteche, CM
影响因子：
10.5
作者：
Gilfillan, GD;Straub, T;Becker, PB
通讯作者：
Becker, PB
影响因子：
9.8
作者：
Hense, Winfried;Baines, John F.;Parsch, John
通讯作者：
Parsch, John