Involvement of miR-20a in promoting gastric cancer progression by targeting early growth response 2 (EGR2).
Involvement of miR-20a in promoting gastric cancer progression by targeting early growth response 2 (EGR2).
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DOI：
10.3390/ijms140816226
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发表时间：
2013-08-06
影响因子：
5.6
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Li X;Zhang Z;Yu M;Li L;Du G;Xiao W;Yang H
胃癌（Gastric cancer，GC）是最常见的恶性肿瘤之一，在东亚地区发病率较高. microRNAs（miRNAs）在胃癌的发生发展中起着重要作用。miR-20 a在胃癌中升高，而miR-20 a的潜在功能知之甚少。在GC组织和细胞系中检测miR-20 a表达。检测miR-20 a对胃癌细胞生长、迁移、侵袭和化疗耐药性的影响。采用荧光素酶报告基因分析和Western blot检测miR-20 a的作用靶点。miR-20 a在胃癌组织和胃癌细胞系中表达增加。miR-20 a可促进胃癌细胞的生长、迁移和侵袭，增强胃癌细胞对顺铂和多西他赛的耐药性。荧光素酶活性和Western blot证实miR-20 a负调控EGR 2的表达。EGFR 2的过表达显著减弱了miR-20 a的致癌作用。miR-20 a通过调节EGR 2信号通路参与胃癌的发生。
Gastric cancer (GC) is one of the most common cancers, with high incidences in East Asia. microRNAs (miRNAs) play essential roles in the carcinogenesis of GC. miR-20a was elevated in GC, while the potential function of miR-20a was poorly understood. miR-20a expression was examined in GC tissues and cell lines. The effects of miR-20a on the growth, migration, invasion, and chemoresistance of GC cells were examined. Luciferase reporter assay and Western blot were used to screen the target of miR-20a. miR-20a was increased in GC tissues and cell lines. miR-20a promoted the growth, migration and invasion of GC cells, enhanced the chemoresistance of GC cells to cisplatin and docetaxel. Luciferase activity and Western blot confirmed that miR-20a negatively regulated EGR2 expression. Overexpression of EGR2 significantly attenuated the oncogenic effect of miR-20a. miR-20a was involved in the carcinogenesis of GC through modulation of the EGR2 signaling pathway.
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