Gut Microbiome of Children and Adolescents With Primary Sclerosing Cholangitis in Association With Ulcerative Colitis.
Gut Microbiome of Children and Adolescents With Primary Sclerosing Cholangitis in Association With Ulcerative Colitis.
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DOI：
10.3389/fimmu.2020.598152
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发表时间：
2020
影响因子：
7.3
通讯作者：
Taddei CR
中科院分区：
文献类型：
--
作者：
Cortez RV;Moreira LN;Padilha M;Bibas MD;Toma RK;Porta G;Taddei CR
少数研究报道了原发性硬化性胆管炎（PSC）和溃疡性结肠炎（UC）儿科患者肠道微生物组组成和多样性的关系。在这项横断面研究中，我们从一家三级医院的儿科胃肠病和肝病门诊中选择了年龄小于19岁的患者，以描述与UC相关或无关的PSC儿科患者的肠道微生物组。将患者分为PSC、PSC+UC和UC诊断。从每名患者（n=30）和健康亲属/邻居（n=23）收集粪便样品。使用MiSeq（Illumina）平台评估微生物组组成。在PSC和PSC+UC组之间发现微生物组成的差异。韦荣球菌属和巨球菌属的相对丰度随患者诊断时的年龄而增加。韦荣氏球菌在处于疾病活动状态的患者中也有所增加。两种属均与总胆红素和γ-谷氨酰转移酶呈正相关。总之，疾病、年龄和疾病活动状态似乎会影响肠道微生物组，突出了患者肠道微生物组特征的差异，这取决于诊断时的年龄。我们还发现PSC和PSC+UC患者中韦荣氏球菌的增加，PSC+UC患者中微生态失调与较高的γ-谷氨酰转移酶和总胆红素呈正相关。我们的研究结果在PSC患者的诊断、预后和未来的治疗前景方面是有希望的。
Few studies reported the relation of intestinal microbiome composition and diversity in pediatric patients with primary sclerosing cholangitis (PSC) and ulcerative colitis (UC). In this cross-sectional study, we selected patients younger than 19 years old from the pediatric gastroenterology and hepatology outpatient clinic of a tertiary hospital to describe the intestinal microbiome of pediatric patients with PSC associated or not to UC. Patients were divided in PSC, PSC+UC, and UC diagnosis. A stool sample was collected from each patient (n=30) and from a healthy relative/neighbor (n=23). The microbiome composition was assessed using MiSeq (Illumina) platform. Differences in microbial composition were found between PSC and PSC+UC groups. The relative abundance of Veillonella and Megasphaera genera were increased depending on patients’ age at diagnosis. Veillonella was also increased in patients who were in an active status of the disease. Both genera were positively correlated to total bilirubin and gamma-glutamyl transferase. As a conclusion, the disease, the age and the disease activity status seem to influence the intestinal microbiome, highlighting the difference of intestinal microbiome profile for patients depending on age at diagnosis. We also showed an increase of Veillonella in patients with PSC and PSC+UC, and a positive correlation of dysbiosis and higher gamma-glutamyl transferase and total bilirubin in PSC+UC patients. Our findings are promising in the diagnosis, prognosis, and future therapeutic perspectives for PSC patients.
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影响因子：
5.7
作者：
Knapp S;Brodal C;Peterson J;Qi F;Kreth J;Merritt J
通讯作者：
Merritt J
影响因子：
2.1
作者：
Lee, Way Seah;Karthik, Sivaramakrishnan Venkatesh;Aw, Marion Margaret
通讯作者：
Aw, Marion Margaret
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
29.7
作者：
Kaser A;Zeissig S;Blumberg RS
通讯作者：
Blumberg RS
影响因子：
6.7
作者：
Mueller, Tobias;Beutler, Claudia;Podolsky, Daniel K.
通讯作者：
Podolsky, Daniel K.