Defining the molecular character of the developing and adult kidney podocyte.
Defining the molecular character of the developing and adult kidney podocyte.
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DOI：
10.1371/journal.pone.0024640
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Potter SS
中科院分区：
文献类型：
--
作者：
Brunskill EW;Georgas K;Rumballe B;Little MH;Potter SS
足细胞是一种显著的细胞类型，它包裹着肾小球的毛细血管。虽然起源于中胚层，但它发出轴突样的突起，包裹在毛细血管周围。这些突起延伸出更细的突起，即足突，足突相互交错，在足突之间留下了肾小球滤液必须通过的横隔。足细胞是一个非常感兴趣的主题，因为它们在肾脏发育和疾病中的关键作用。在这份报告中，我们确定和表征了一种新的转基因小鼠系，MafB-GFP，它特异性地标记了肾脏足细胞从非常早期的发展阶段。然后，这些小鼠用于促进基于荧光激活细胞分选的来自E13.5和E15.5的胚胎以及成体的足细胞的纯化。然后使用微阵列来全面定义足细胞在这些不同发育阶段的基因表达状态。一幅引人注目的图像出现了，确定了多组基因，这些基因建立了足细胞的神经元，肌肉和吞噬功能。完整的组合编码的转录因子，创造足细胞的特点，并定义了全球名单的生长因子和受体，他们表达。首次建立了体内足细胞的完整分子特征。活性分子功能和生物学过程进一步定义了它们独特的特征组合。这些结果提供了发育和成年足细胞基因表达模式的资源图谱，将有助于指导这些令人难以置信的细胞的进一步研究。
The podocyte is a remarkable cell type, which encases the capillaries of the kidney glomerulus. Although mesodermal in origin it sends out axonal like projections that wrap around the capillaries. These extend yet finer projections, the foot processes, which interdigitate, leaving between them the slit diaphragms, through which the glomerular filtrate must pass. The podocytes are a subject of keen interest because of their key roles in kidney development and disease. In this report we identified and characterized a novel transgenic mouse line, MafB-GFP, which specifically marked the kidney podocytes from a very early stage of development. These mice were then used to facilitate the fluorescent activated cell sorting based purification of podocytes from embryos at E13.5 and E15.5, as well as adults. Microarrays were then used to globally define the gene expression states of podocytes at these different developmental stages. A remarkable picture emerged, identifying the multiple sets of genes that establish the neuronal, muscle, and phagocytic properties of podocytes. The complete combinatorial code of transcription factors that create the podocyte was characterized, and the global lists of growth factors and receptors they express were defined. The complete molecular character of the in vivo podocyte is established for the first time. The active molecular functions and biological processes further define their unique combination of features. The results provide a resource atlas of gene expression patterns of developing and adult podocytes that will help to guide further research of these incredible cells.
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