A new method to prepare multicellular spheroids in cancer cell lines using a thermo-reversible gelation polymer.
A new method to prepare multicellular spheroids in cancer cell lines using a thermo-reversible gelation polymer.
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一种使用热可逆凝胶聚合物在癌细胞系中制备多细胞球体的新方法。
DOI：
10.1046/j.1525-1594.2003.07131.x
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发表时间：
2003
影响因子：
2.4
通讯作者：
S. Kubota
中科院分区：
文献类型：
--
作者：
S. Tsukikawa;H. Matsuoka;Y. Kurahashi;Yasushi Konno;Kohichi Satoh;Ryotaro Satoh;A. Isogai;Kanako Kimura;Yasuharu Watanabe;S. Nakano;J. Hayashi;S. Kubota
本研究的目的是利用溶胶-凝胶过渡相随温度可逆变化的热可逆凝胶化聚合物在三维培养体系中进行培养。已经观察到人癌细胞形成多细胞球体，而成纤维细胞在含有该聚合物的培养基中缓慢发育成小球体。该聚合物用作培养材料具有如下优点：第一，癌细胞在该聚合物的水溶液中三维生长;第二，通过简单地冷却温度，容易在该聚合物的水溶液中收获细胞或球状体;第三，包含该聚合物的培养基是半透明的，使得可以通过相差显微镜观察到细胞或球状体。因此，我们得出结论，这种聚合物是一个非常有用的材料三维文化。
The purpose of this study is to utilize the thermo-reversible gelation polymer in which the sol-gel transitting phase is reversibly changed by temperature in a three-dimensional culture system. Human cancer cells have been observed to form multicellular spheroids, whereas fibroblasts slowly develop into small spheroids with the culture medium including this polymer. This polymer has some advantages for use as a culture material, as follows: first, cancer cells grow three-dimensionally in the aqueous solution of this polymer; second, it is easy to harvest cells or spheroids in the aqueous solution of this polymer by simply cooling down the temperature; and third, the culture medium including this polymer is so translucent that the cells or spheroids can be observed through a phase-contrast microscope. We thus conclude that this polymer is a very useful material for three-dimensional cultures.
影响因子：
56.9
作者：
SUTHERLAND, RM
通讯作者：
SUTHERLAND, RM