Chemotaxis of human gingival epithelial cells to laminin. A mechanism for epithelial cell apical migration.
Chemotaxis of human gingival epithelial cells to laminin. A mechanism for epithelial cell apical migration.
复制标题
人牙龈上皮细胞对层粘连蛋白的趋化性。
DOI：
10.1902/jop.1986.57.5.311
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发表时间：
1986
影响因子：
4.3
通讯作者：
R. Lyall
中科院分区：
文献类型：
--
作者：
V. Terranova;R. Lyall
层粘连蛋白，一种大的糖蛋白（Mr = 10（6））和基底膜的主要成分，在这里被证明是一种有效的化学引诱剂，为人类牙龈上皮细胞。改良Boyden小室法测定层粘连蛋白刺激牙龈上皮细胞的趋化性和趋化性。这种作用似乎是层粘连蛋白受体介导的。牙龈上皮细胞显示结合层粘连蛋白（Kd = 2.0 nM），每个细胞具有10，000至30，000个结合位点。抗层粘连蛋白抗体抑制层粘连蛋白结合，抑制上皮细胞对层粘连蛋白的趋化反应，而抗纤连蛋白则无此作用。纤连蛋白是不作为一个有效的化学引诱层粘连蛋白。还测试了其他生物反应调节剂;其中，IV型胶原蛋白和表皮生长因子作为趋化剂是有活性的，尽管在诱导趋化性方面不如层粘连蛋白有效。这些数据表明，层粘连蛋白和基底膜的其他成分可能是重要的，在调节牙龈上皮细胞的迁移和生长。
Laminin, a large glycoprotein (Mr = 10(6)) and a major component of basement membrane, is shown here to be a potent chemoattractant for human gingival epithelial cells. Laminin stimulated chemotaxis and chemokinesis of gingival epithelial cells in the modified Boyden chamber assay. This effect appeared to be laminin receptor mediated. Gingival epithelial cells were shown to bind laminin (Kd = 2.0 nM) with 10,000 to 30,000 binding sites per cell. Antilaminin antibody, which inhibited laminin binding, inhibited the chemotactic response of epithelial cells to laminin, while antifibronectin was without effect. Fibronectin was not as potent a chemoattractant as laminin. Other biological response modifiers were also tested; of these, Type IV collagen and epidermal growth factor were active as chemoattractants, although not as effective in inducing chemotaxis as laminin. The data indicate that laminin and other components of basement membrane may be important in regulating the migration and growth of gingival epithelial cells.
影响因子：
2.7
作者：
AKERS, RM;MOSHER, DF;LILIEN, JE
通讯作者：
LILIEN, JE
胚胎角膜上皮与外源胶原、层粘连蛋白和纤连蛋白的相互作用：内源蛋白质合成的作用。
影响因子：
2.7
作者：
Sugrue,SP;Hay,ED
通讯作者：
Hay,ED