Phylogenetic analysis of isolates of Beet necrotic yellow vein virus collected worldwide.
Phylogenetic analysis of isolates of Beet necrotic yellow vein virus collected worldwide.
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全球收集的甜菜坏死黄脉病毒分离株的系统发育分析。
DOI：
10.1099/vir.0.81167-0
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
O. Lemaire
中科院分区：
文献类型：
--
作者：
A. Schirmer;D. Link;V. Cognat;B. Moury;M. Beuve;A. Meunier;C. Bragard;D. Gilmer;O. Lemaire
对136只感染甜菜坏死黄静脉病毒（BNYVV, Benyvirus）的甜菜进行了分子多样性研究。分析rna -2编码的CP、rna -3编码的p25和rna -5编码的p26蛋白的核苷酸序列。由此产生的系统发育树允许将BNYVV分类为显示病毒簇与地理起源之间相关性的组。通过最大似然模型估计同义和非同义替代率之间的比值（omega）来衡量这三个序列的选择约束。结果表明，选择性约束对蛋白质施加了不同的作用。CP最保守，平均omega值在0.12 ~ 0.15之间，而p26受约束较少，平均omega值在0.20 ~ 0.33之间。在p26的3个氨基酸位置检测到选择性，ω值约为5.0。p25序列的平均omega值最高（0.36-1.10），对14个氨基酸具有强阳性选择（omega=4.7-54.7），特别是对氨基酸68，其omega值是迄今为止在植物病毒中遇到的最高的。
A study of molecular diversity was carried out on 136 sugar beets infected with Beet necrotic yellow vein virus (BNYVV, Benyvirus) collected worldwide. The nucleotide sequences of the RNA-2-encoded CP, RNA-3-encoded p25 and RNA-5-encoded p26 proteins were analysed. The resulting phylogenetic trees allowed BNYVV to be classified into groups that show correlations between the virus clusters and geographic origins. The selective constraints on these three sequences were measured by estimating the ratio between synonymous and non-synonymous substitution rates (omega) with maximum-likelihood models. The results suggest that selective constraints are exerted differently on the proteins. CP was the most conserved, with mean omega values ranging from 0.12 to 0.15, while p26 was less constrained, with mean omega values ranging from 0.20 to 0.33. Selection was detected in three amino acid positions of p26, with omega values of about 5.0. The p25 sequences presented the highest mean omega values (0.36-1.10), with strong positive selection (omega=4.7-54.7) acting on 14 amino acids, and particularly on amino acid 68, where the omega value was the highest so far encountered in plant viruses.
每个位点的同义和非同义替换数量的方差和协方差。
影响因子：
10.7
作者：
Ota,T;Nei,M
通讯作者：
Nei,M
影响因子：
6.5
作者：
J. Felsenstein
通讯作者：
J. Felsenstein