PaxA, but not PaxC, is required for cnidocyte development in the sea anemone Nematostella vectensis.
PaxA, but not PaxC, is required for cnidocyte development in the sea anemone Nematostella vectensis.
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DOI：
10.1186/s13227-017-0077-7
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发表时间：
2017
期刊：
影响因子：
4.1
通讯作者：
Martindale MQ
中科院分区：
文献类型：
--
作者：
Babonis LS;Martindale MQ
Pax基因是一个保守的转录因子家族，调节发育形态发生的许多方面，特别是包括眼睛在内的外胚层感觉结构的发育。Nematostella vectensis，星海葵，有许多Pax的直系同源物，其中许多在胚胎发育早期表达。Pax基因在这种无眼刺胞动物中的功能尚不清楚。在这里，我们表明PaxA而不是PaxC在N. vectensis。PaxA的敲低导致发育中的刺胞的损失和许多刺胞特异性基因的下调，包括转录因子Mef 2的变体。我们还表明，Mef 2在PaxA表达细胞的一个子集中的共表达与第二谱系的nidocytes的发育相关，并表明敲低神经祖细胞基因SoxB 2导致PaxA，Mef 2和几个nidocytes特异性基因的表达下调。由于PaxA在巢细胞发育过程中的任何时候都不与SoxB 2共表达，因此我们提出了一种简单的巢发生模型，即表达SoxB 2的祖细胞群经过分裂产生表达PaxA的巢细胞，其中一些也表达Mef 2。PaxA在水螅体中的刺突细胞发育中的作用尚未研究，但SoxB 2在调节Nematostella和Hydracactinia echinata中产生神经元和刺突细胞的祖细胞的命运中的保守作用表明，这种SoxB 2/PaxA途径可能在刺胞动物中是保守的。
Pax genes are a family of conserved transcription factors that regulate many aspects of developmental morphogenesis, notably the development of ectodermal sensory structures including eyes. Nematostella vectensis, the starlet sea anemone, has numerous Pax orthologs, many of which are expressed early during embryogenesis. The function of Pax genes in this eyeless cnidarian is unknown. Here, we show that PaxA, but not PaxC, plays a critical role in the development of cnidocytes in N. vectensis. Knockdown of PaxA results in a loss of developing cnidocytes and downregulation of numerous cnidocyte-specific genes, including a variant of the transcription factor Mef2. We also demonstrate that the co-expression of Mef2 in a subset of the PaxA-expressing cells is associated with the development with a second lineage of cnidocytes and show that knockdown of the neural progenitor gene SoxB2 results in downregulation of expression of PaxA, Mef2, and several cnidocyte-specific genes. Because PaxA is not co-expressed with SoxB2 at any time during cnidocyte development, we propose a simple model for cnidogenesis whereby a SoxB2-expressing progenitor cell population undergoes division to give rise to PaxA-expressing cnidocytes, some of which also express Mef2. The role of PaxA in cnidocyte development among hydrozoans has not been studied, but the conserved role of SoxB2 in regulating the fate of a progenitor cell that gives rise to neurons and cnidocytes in Nematostella and Hydractinia echinata suggests that this SoxB2/PaxA pathway may well be conserved across cnidarians.
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DOI：
10.1073/pnas.1003256107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Hwang, Jung Shan;Takaku, Yasuharu;Gojobori, Takashi
通讯作者：
Gojobori, Takashi
影响因子：
0.8
作者：
FRANK, P;BLEAKNEY, JS
通讯作者：
BLEAKNEY, JS
影响因子：
4.6
作者：
Genikhovich, Grigory;Technau, Ulrich
通讯作者：
Technau, Ulrich
影响因子：
3.9
作者：
Hoshiyama, D;Suga, H;Miyata, T
通讯作者：
Miyata, T
影响因子：
3.7
作者：
Brinkman, Diane L.;Aziz, Ammar;Mulvenna, Jason
通讯作者：
Mulvenna, Jason