Discovery of defense- and neuropeptides in social ants by genome-mining.
Discovery of defense- and neuropeptides in social ants by genome-mining.
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DOI：
10.1371/journal.pone.0032559
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Muttenthaler M
中科院分区：
文献类型：
--
作者：
Gruber CW;Muttenthaler M
天然肽的数量和多样性存在于所有生物体中，但分析它们的肽组往往是困难的。考虑到天然产物药物的发现，我们设计了一种基因组挖掘方法，以确定防御和神经肽的社会蚂蚁从阿塔cephalotes（切叶蚂蚁），Camponotus floridanus（木匠蚂蚁）和Harpegnathos saltator（基础属）的基因组。许多肽编码基因的防御肽，特别是防御素，和神经肽或调节肽激素，如allatostatin和速激肽，被确定和分析。最有趣的是，我们注释了编码催产素/加压素相关肽（催产素）及其假定受体的基因。这是蚂蚁（蚁科）中存在这种九肽激素系统的第一个证据，并支持最近在赤拟谷盗（红粉甲虫）和丽蝇蛹集蜂（寄生蜂）中的发现，因此它局限于一些基底全变态昆虫。与此相反，蜜蜂（Apis mellifera）中催产素激素系统的缺失为进一步研究催产素/加压素相关肽（催产素九肽家族）及其受体在社会昆虫中的分子进化和生理功能奠定了基础。特别是antinotocin和防御素肽序列的鉴定将为未来寻求具有治疗价值的有效和选择性先导化合物的药理学表征提供基础。
Natural peptides of great number and diversity occur in all organisms, but analyzing their peptidome is often difficult. With natural product drug discovery in mind, we devised a genome-mining approach to identify defense- and neuropeptides in the genomes of social ants from Atta cephalotes (leaf-cutter ant), Camponotus floridanus (carpenter ant) and Harpegnathos saltator (basal genus). Numerous peptide-encoding genes of defense peptides, in particular defensins, and neuropeptides or regulatory peptide hormones, such as allatostatins and tachykinins, were identified and analyzed. Most interestingly we annotated genes that encode oxytocin/vasopressin-related peptides (inotocins) and their putative receptors. This is the first piece of evidence for the existence of this nonapeptide hormone system in ants (Formicidae) and supports recent findings in Tribolium castaneum (red flour beetle) and Nasonia vitripennis (parasitoid wasp), and therefore its confinement to some basal holometabolous insects. By contrast, the absence of the inotocin hormone system in Apis mellifera (honeybee), another closely-related member of the eusocial Hymenoptera clade, establishes the basis for future studies on the molecular evolution and physiological function of oxytocin/vasopressin-related peptides (vasotocin nonapeptide family) and their receptors in social insects. Particularly the identification of ant inotocin and defensin peptide sequences will provide a basis for future pharmacological characterization in the quest for potent and selective lead compounds of therapeutic value.
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影响因子：
3.8
作者：
Aikins, Michael J.;Schooley, David A.;Park, Yoonseong
通讯作者：
Park, Yoonseong
影响因子：
2.8
作者：
Davies, NW;Wiese, MD;Browne, SGA
通讯作者：
Browne, SGA
DOI：
10.1073/pnas.0605858103
发表时间：
2006-11-28
影响因子：
11.1
作者：
Brady, Sean G.;Schultz, Ted R.;Ward, Philip S.
通讯作者：
Ward, Philip S.
影响因子：
4.8
作者：
Clark, Richard J.;Fischer, Harald;Craik, David J.
通讯作者：
Craik, David J.
影响因子：
3.5
作者：
Chini, B;Mouillac, B;Barberis, C
通讯作者：
Barberis, C