Hsp60 response in experimental and human temporal lobe epilepsy.
Hsp60 response in experimental and human temporal lobe epilepsy.
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DOI：
10.1038/srep09434
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发表时间：
2015-03-24
影响因子：
4.6
通讯作者：
Macario AJ
中科院分区：
文献类型：
--
作者：
Marino Gammazza A;Colangeli R;Orban G;Pierucci M;Di Gennaro G;Lo Bello M;D'Aniello A;Bucchieri F;Pomara C;Valentino M;Muscat R;Benigno A;Zummo G;de Macario EC;Cappello F;Di Giovanni G;Macario AJ
线粒体伴侣蛋白Hsp60是一种普遍存在的具有多种作用的分子，组成型表达并可由氧化应激诱导。在大脑中，Hsp60广泛分布，并与包括癫痫在内的神经系统疾病有关。线粒体和氧化应激在颞叶癫痫（TLE）的癫痫发生中的作用已被提出。在这里，我们调查了热休克蛋白60在TLE使用动物和人类样本的参与。在颞叶癫痫大鼠模型中，通过免疫印迹和免疫组织化学测定，海马中的Hsp60免疫反应性增加。Hsp60在癫痫大鼠海马齿状回神经元胞体、神经元胞体及海马本体（CA3、CA1）也有表达。我们还确定了循环水平的热休克蛋白60在癫痫动物和TLE患者使用ELISA。癫痫大鼠循环中Hsp60水平高于对照组。同样，患者癫痫发作后血浆Hsp60水平高于癫痫发作前和对照组。这些结果表明，Hsp60在动物和TLE患者的受影响组织中增加，并在血浆中响应癫痫发作，并指出它作为海马应激的生物标志物可能用于诊断和患者管理。
The mitochondrial chaperonin Hsp60 is a ubiquitous molecule with multiple roles, constitutively expressed and inducible by oxidative stress. In the brain, Hsp60 is widely distributed and has been implicated in neurological disorders, including epilepsy. A role for mitochondria and oxidative stress has been proposed in epileptogenesis of temporal lobe epilepsy (TLE). Here, we investigated the involvement of Hsp60 in TLE using animal and human samples. Hsp60 immunoreactivity in the hippocampus, measured by Western blotting and immunohistochemistry, was increased in a rat model of TLE. Hsp60 was also increased in the hippocampal dentate gyrus neurons somata and neuropil and hippocampus proper (CA3, CA1) of the epileptic rats. We also determined the circulating levels of Hsp60 in epileptic animals and TLE patients using ELISA. The epileptic rats showed circulating levels of Hsp60 higher than controls. Likewise, plasma post-seizure Hsp60 levels in patients were higher than before the seizure and those of controls. These results demonstrate that Hsp60 is increased in both animals and patients with TLE in affected tissues, and in plasma in response to epileptic seizures, and point to it as biomarker of hippocampal stress potentially useful for diagnosis and patient management.
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影响因子：
3.8
作者：
Gammazza, Antonella Marino;Rizzo, Manfredi;Cappello, Francesco
通讯作者：
Cappello, Francesco
影响因子：
6.6
作者：
Calabrese, Vittorio;Sultana, Rukhsana;Butterfield, D. A.
通讯作者：
Butterfield, D. A.
影响因子：
3.3
作者：
Kim, Y. -J.;Kim, J. -Y.;Kang, T. -C.
通讯作者：
Kang, T. -C.
影响因子：
3.3
作者：
GASS, P;PRIOR, P;KIESSLING, M
通讯作者：
KIESSLING, M
影响因子：
3.7
作者：
Campanella C;Bucchieri F;Merendino AM;Fucarino A;Burgio G;Corona DF;Barbieri G;David S;Farina F;Zummo G;de Macario EC;Macario AJ;Cappello F
通讯作者：
Cappello F