The Haemophilus Cryptic Genospecies Cha Adhesin Has at Least Two Variants That Differ in Host Cell Binding, Bacterial Aggregation, and Biofilm Formation Properties
The Haemophilus Cryptic Genospecies Cha Adhesin Has at Least Two Variants That Differ in Host Cell Binding, Bacterial Aggregation, and Biofilm Formation Properties
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嗜血杆菌隐性基因种 Cha 粘附素至少有两种在宿主细胞结合、细菌聚集和生物膜形成特性方面不同的变体
DOI：
10.1128/jb.01409-13
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发表时间：
2014
影响因子：
3.2
通讯作者：
J. S. St. Geme
中科院分区：
文献类型：
--
作者：
J. Mccann;A. Sheets;S. Grass;J. S. St. Geme
摘要隐型嗜血杆菌(HCG)可引起孕妇和产后妇女生殖道感染和新生儿呼吸道感染。HCG中主要的表面粘附素称为CHA，它介导细菌对培养的人上皮细胞的黏附。在这项研究中，我们报告了CHA的两个抗原性不同的变体，称为Cha1和Cha2。这些变异体在不同的菌株中由相同的遗传位点编码，具有几乎相同的N端输出和C端表面锚定结构域，但内部粘附域明显不同。根据对一株表达表面相关CHA1和表面相关CHA2的实验室流感嗜血杆菌的衍生物的比较，CHA1介导了对培养的人上皮细胞的更高水平的黏附，CHA2介导了更高水平的对非生物表面的黏附。我们推测，CHA1和CHA2内部区域的变异会导致结合特异性或结合亲和力的变化，并可能与在定殖和疾病期间对不同宿主环境的适应有关。
ABSTRACT The Haemophilus cryptic genospecies (HCG) causes genital tract infections in pregnant and postpartum women and respiratory infections in neonates. The major surface adhesin in HCG is called Cha, which mediates bacterial adherence to cultured human epithelial cells. In this study, we report that there are two antigenically distinct variants of Cha, dubbed Cha1 and Cha2. These variants are encoded by the same genetic locus in diverse strains and have nearly identical N-terminal export and C-terminal surface anchoring domains but significantly different internal adhesive domains. Based on the comparison of derivatives of a laboratory strain of Haemophilus influenzae expressing either surface-associated Cha1 or surface-associated Cha2, Cha1 mediates a higher level of adherence to cultured human epithelial cells and Cha2 mediates a higher level of adherence to abiotic surfaces. We hypothesize that variation in the Cha1 and Cha2 internal region results in changes in binding specificity or binding affinity and may be associated with adaptation to different host environments during colonization and disease.
DOI：
10.1073/pnas.1211872110
发表时间：
2012-12-18
影响因子：
11.1
作者：
Hartmann, Marcus D.;Grin, Iwan;Alvarez, Birte Hernandez
通讯作者：
Alvarez, Birte Hernandez
泌尿生殖系统、孕产妇和新生儿流感嗜血杆菌分离株：异常毒力血清学不可分型克隆家族的鉴定和新嗜血杆菌种的证据。
DOI：
10.1099/00221287-136-7-1203
发表时间：
1990
期刊：
Journal of general microbiology
影响因子：
--
作者：
Quentin,R;Goudeau,A;WallaceJr,RJ;Smith,AL;Selander,RK;Musser,JM
通讯作者：
Musser,JM