Functional biomimetic analogs help remineralize apatite-depleted demineralized resin-infiltrated dentin via a bottom-up approach.
Functional biomimetic analogs help remineralize apatite-depleted demineralized resin-infiltrated dentin via a bottom-up approach.
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DOI：
10.1016/j.actbio.2009.12.052
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发表时间：
2010-07
影响因子：
9.7
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
--
作者：
Kim, Jongryul;Arola, Dwayne D.;Gu, Lisha;Kim, Young Kyung;Mai, Sui;Liu, Yan;Pashley, David H.;Tay, Franklin R.
天然生物矿物是通过自下而上的纳米颗粒介导的矿化机制，通过亚稳的无定形前体相形成的。使用酸蚀刻的人牙本质模型来创建完全脱矿的胶原蛋白基质层，基于使用双重仿生类似物，已经开发了生物启发的矿化方案。这些类似物有助于螯合流体无定形磷酸钙纳米前体，并用作引导胶原原纤维内的均匀磷灰石成核的模板。通过采用该方案对粘合树脂结合的完全脱矿牙本质进行再矿化，我们已经能够在树脂不完全渗透的完全脱矿胶原基质中再沉积原纤维内和原纤维外磷灰石。本研究利用一系列完全和部分脱矿的牙本质胶原基质，以进一步验证使用含仿生类似物的介质对树脂渗透的部分脱矿的胶原基质进行再矿化的必要性，其中存在残留的种子微晶。在对照标本中，其中仿生类似物不存在于矿化介质中，矿化只能在部分脱矿的胶原基质中看到，可能是通过自上而下的结晶方法进行外延生长。相反，在仿生类似物存在下的再矿化介质中，完全脱矿的胶原蛋白基质通过自下而上的结晶机制的纤维内再矿化可以另外被识别。后者的特征在于原纤维内矿物质从连续编织的微原纤维电子致密无定形链的早期状态到离散的纳米晶体的转变，并最终转变成胶原原纤维内的更大的结晶血小板。通过双仿生类似物的仿生再矿化有可能被转化为用于挽救失败的树脂-牙本质键合的功能性递送系统。
Natural biominerals are formed through metastable amorphous precursor phases via a bottom-up, nanoparticle-mediated mineralization mechanism. Using an acid-etched human dentin model to create a layer of completely-demineralized collagen matrix, a bio-inspired mineralization scheme has been developed based on the use of dual biomimetic analogs. These analogs help to sequester fluidic amorphous calcium phosphate nanoprecursors and function as templates for guiding homogeneous apatite nucleation within the collagen fibrils. By adopting this scheme for remineralizing adhesive resin-bonded, completely-demineralized dentin, we have been able to redeposit intrafibrillar and extrafibrillar apatites in completely-demineralized collagen matrices that are imperfectly infiltrated by resins. This study utilizes a spectrum of completely- and partially-demineralized dentin collagen matrices to further validate the necessity for using a biomimetic analog-containing medium for remineralizing resin-infiltrated partially-demineralized collagen matrices in which remnant seed crystallites are present. In control specimens in which biomimetic analogs are absent from the remineralization medium, remineralization could only be seen in partially-demineralized collagen matrices probably by epitaxial growth via a top-down crystallization approach. Conversely, in the presence of biomimetic analogs in the remineralization medium, intrafibrillar remineralization of completely-demineralized collagen matrices via a bottom-up crystallization mechanism can additionally be identified. The latter is characterized by the transition of intrafibrillar minerals from an inchoate state of continuously-braided microfibrillar electron-dense amorphous strands to discrete nanocrystals, and ultimately into larger crystalline platelets within the collagen fibrils. Biomimetic remineralization via dual biomimetic analogs has the potential to be translated into a functional delivery system for salvaging failing resin-dentin bonds.
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影响因子：
3.8
作者：
Deshpande, Atul S.;Beniash, Elia
通讯作者：
Beniash, Elia
影响因子：
--
作者：
LINDE, A
通讯作者：
LINDE, A
DOI：
10.1093/protein/9.1.43
发表时间：
1996-01-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
King, G;Brown, EM;Chen, JM
通讯作者：
Chen, JM
影响因子：
3.4
作者：
Chesnick, Ingrid E.;Mason, Jeffrey T.;Potter, Kimberlee
通讯作者：
Potter, Kimberlee
影响因子：
62.1
作者：
Gower, Laurie B.
通讯作者：
Gower, Laurie B.